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Optimization of Culture Conditions and Extraction and Analysis of Polysaccharides of Hypsizigus Marmoreus

Author: WangGeng
Tutor: HuKaiHui;YeRuiHong
School: Fujian Agriculture and Forestry University
Course: Biochemistry and Molecular Biology
Keywords: True cornucopiae Optimization of culture conditions Polysaccharide extracted Process optimization Inhibitory effect
CLC: S646
Type: Master's thesis
Year: 2009
Downloads: 140
Quote: 1
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True cornucopiae (Hypsizigus marmoreus) the edible fungus with good health. China from the 1980s to carry out the study of the biological characteristics and cultivation conditions. Really cornucopiae True cornucopiae nutritious, including the eight essential amino acids, including lysine, arginine content is higher than the general mushrooms, marmoreus polysaccharide contains many important biologically active substances, such as real cornucopiae polysaccharide antifungal protein glial binding protein, the ergosterol and Hypsizigus mushroom polysaccharides have good anti-tumor activity. Shake flask cultures submerged culture conditions for real cornucopiae really the cornucopiae good health functions and broad market prospects, namely speed, temperature, pH, inoculum size, bottled amount of training time. The results show that, really cornucopiae submerged culture conditions: speed 120 r / min, a temperature of 25 ° C, pH 4.0-7.0 (generally do not need to adjust the pH), inoculum size is about 10% (250 mL conical flask , 100 mL bottle volume), bottled capacity of 80 mL (250 ml Erlenmeyer flask), the training time is 7 days. Verified by experiments that the results are credible. Mushroom Polysaccharide Extracts Hypsizigus usually hot water extraction, solvent extraction and enzymatic extraction of the status quo, the hot water extraction innovative improvements. Although the hot water extraction yield enzymatic low, but the process is simple, easy to operate and economical. So this article using hot water extraction method to extract the true cornucopiae polysaccharide. Single factor test in order to get the optimum conditions for extraction Hypsizigus mushroom polysaccharides, extraction temperature, extraction time, the ratio of liquid to solid, the role of alcohol analysis and extraction times polysaccharide extracted five factors, followed polysaccharide extracted orthogonal test come true cornucopiae polysaccharide best combination, according to the test results through the use of spss statistical software. The results showed that when the extraction temperature was 90 ° C, the extraction time was 3h, liquid to solid ratio of 30:1, 2 times the amount of ethanol extraction times 2 times, marmoreus polysaccharide extract the highest rate. Antibacterial activity Preliminary real Kyi the Mycelial and fruiting polysaccharide, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae, Aspergillus niger and Rhizopus tested strains by disk agar determination of the Mycelial and fruiting bodies polysaccharide in Hypsizigus antibacterial strength of tested strains. The results show that the true Ji Mycelial Polysaccharide between 10 ~ 5mg/mL, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae have certain inhibition, minimum inhibitory concentration Rhizopus and Aspergillus niger no inhibitory effect. True cornucopiae better nutritional and medicinal value, but its development depth few really cornucopiae polysaccharide extraction rate is generally not high, so there are a lot of improvements on the true cornucopiae extraction of polysaccharides space, such as walking in front of Lentinan extraction method and extraction rate can be considered applicable to real cornucopiae Extraction, in order to improve the extraction rate of polysaccharides.

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CLC: > Agricultural Sciences > Gardening > Vegetable gardening > Fungi ( mushroom )
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