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Vitro drug-induced method to study the role of cAMP signaling pathway hardshell clams 5 - serotonin (5-hydroxytryptamine ,5-HT)-induced oocyte maturation process. The results showed that ,5-HT (0.01-100μM) were able to significantly induce clams oocytes maturation. Phosphodiesterase inhibitors - caffeine, theophylline and IBMX (3 - isobutyl-1 - methylxanthine) alone inhibit oocyte spontaneous maturation, but the effect was not significant. 10 mM caffeine and theophylline and 5 mM IBMX of 5-HT-induced effects can be significantly suppressed. dbcAMP (bis butyryl cyclic adenosine monophosphate) is not only possible to suppress the spontaneous maturation of oocytes, but can also inhibit the 5-HT induced maturation. Therefore, the cAMP signaling pathway involved in the 5-HT-induced oocyte maturation process of hard clams, and the signaling pathway plays a role in the negative regulation. Activator / inhibitor of PLC (phospholipase C) and PKC (protein kinase C) of 5 - serotonin-induced oocyte maturation. A high concentration of neomycin (PLC inhibitor) can inhibit 5-HT induced the maturation of the oocytes, while the DMBA-(9,10-Dimethy-1 ,2-benzanthracene, 9,10 - two methylcholanthrene, PLC activator), it is possible to promote maturation. PMA (phorbol 12-myristate 13-acetate and phorbol myristic acid acetate, PKC activator) can be suppressed for the maturity of 5-HT induced, while Spingosine (PKC inhibitor) can promote the maturation of the oocytes. Thus speculated ,5-HT-induced oocyte maturation requires activation of the phosphatidylinositol signaling pathway. PLC lower concentration of 5-HT induced oocyte maturation can be suppressed; the reduced PKC concentration will promote the maturation of the oocytes. Therefore, in Mercenaria oocyte maturation process, PLC play a role in the promotion (DAG diacyloxy muscle glycerol)-PKC pathway since inhibitory role. High concentrations of extracellular Ca 2 to promote the hard-shell clam oocytes mature the of Ca 2 ionophore A23187 can also promote the hard-shell clam oocytes mature. 1-100μM Isoptin (Verapamil, calcium channel blockers) can inhibit the maturation of oocytes, 100μM of Verapamil completely inhibited mature. The above results show that extracellular Ca 2 Mercenaria oocytes mature is required, but also play a catalytic role of oocyte maturation. Trifluoperazine (TFP, Ca 2 with the CaM-binding antagonists) oocyte maturation can be suppressed, and a high concentration of trifluoperazine (1mM) can completely inhibit the maturation of the oocytes. CaM play a catalytic role of oocyte maturation. A visible, of Ca , 2 through the interaction with CaM jointly play the role of promoting hard-shell clam oocytes mature. 5-HT-induced maturation of oocytes can complete the fertilization process, the process of fertilization, and larval development is similar to the case with the normal developmental process of fertilization, with no significant difference. The high concentration of neomycin can inhibit fertilization process, theophylline and caffeine has no effect on fertility. Thus speculated that phosphatidylinositol signaling pathways involved in Mercenaria oocyte fertilization process, the cAMP signaling pathway may not be involved in the fertilization process. Found the the bivalve Mercenaria gonadal development and our common drunk cockles similar. Hard shell clam oocyte yolk granules from mitochondria, Golgi vacuole, endoplasmic reticulum and micro swallow drink bubble formation.
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