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In this paper, the current problems of the pepper anther culture, on the basis of previous studies, 12 pepper varieties as test materials were pepper anther culture to explore the culture of embryoid induction of the main factors and seedling technology pepper anther culture technology in practice in order to lay a technical foundation. Contents and results are as follows: 1 through 12 kinds of peppers materials buds, anther morphology and of small spores split during the observation contrast, found during the corresponding relations: When the petals twilight, white and green, calyx lobes long or valve slightly longer purple anthers top and three-quarters of the purple part does not exceed anther, the most pollen cells in a early monocyte step aside to dual-core. This criterion is proven to be effective in the latter part of the practice. Nitch, NTH, MS 3 to 9 medium induced comparison: the types of the basal medium for the pepper anther culture embryoid induction is important factors in the present test, MS medium effects are best; basal medium for anther callus induction is not a very important factor, the concentration of plant growth regulators on callus induction of Impact Factor. Plant growth regulator pepper anther culture, the study compared different concentrations of KT and 2,4-D and KT and NAA two groups of medium-induced effects, the results show lower concentrations of plant growth regulator conducive to the induction of embryoids higher concentrations conducive to callus induction; combinations and concentrations of different plant growth regulators, the the embryoid induction rate differences of different genotypes (0.75% to 19.03%); adapt to the type of plant growth regulating agent, and a large difference in concentration, the embryoid induction rate reaches the maximum of the present test in KT 0.05mg / L 2,4-D 0.005 mg / L combinations. Impact of the genotype, 12 were tested by the Institute in the design of eight medium are obtained embryoids embryoids induction rate differences (0.75% to 19.03%) . 5 in the impact of the activated carbon, the test case add 0,0.05%, 0.25%, 0.5% of activated carbon medium embryoid induction comparison results show that the activated carbon can be effectively controlled anther browning and callus degree However, anther culture medium at high concentrations of activated carbon (0.25%, 0.5%) appears mortality; 0.05% activated carbon media can be effectively improve Somatic Embryogenesis of the tested material, the activated carbon concentration of 0.5% of the culture base anther embryoids induction rate dropped significantly. 6 In this study, three tested materials embryoid body morphology was observed and found that not all embryoids can grow into normal plants, the three materials embryoid seedling rate in 39.1 to 45.3% , are very low. Further observed that, the Embryoid occur during the development of four forms: mature embryoids no growth point embryoids, root hairs shaped embryoids stop the development of embryoids in four categories embryoid , only mature embryoids and no growing point embryoids can develop into normal plants, root hairs shaped embryoids and stops growing embryoid not seedling, which is the direct cause of embryoid lower rate of seedling .
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