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Beautiful leaves of autumn leaves shamrock, is an excellent ground cover plants. Extensive management, pest and disease resistance, widely used in the garden. Traditional bulbs breeding methods, it is difficult to meet the market demand, prices have been high, greatly influenced the promotion and application of this plant. This article from autumn leaves shamrock physical climate, photosynthetic characteristics, tissue culture research aspects in order to more comprehensive and in-depth understanding of the characteristics of autumn leaves shamrock this ornamental plants, the optimum growth conditions for the autumn leaves shamrock in the garden planted with applications to provide theoretical guidance; master red leaves shamrock tissue culture techniques to improve the rate of reproduction of the red leaves shamrock, to create the conditions for factory production. In this study, experiments in Hefei, the results are as follows: 1. The autumn leaves shamrock growing season is from March to December, flowering from April to November, the temperature is below 5 ° C or severe frost, the ground part of the death . Autumn leaves shamrock is positive plants, avoid direct sunlight, but the emergence hi warm and humid environment. Sticky soil, the autumn leaves shamrock planting depth should not be more than 20cm, moderate pruning. 2 tissue culture Organogenesis, the best explant material petiole, the best method of disinfection: explant material → water rinse → 70% alcohol disinfection 10 seconds → 0.1% mercuric chloride 5 minutes → The 6 → inoculated sterile water. The basic medium MS, cytokinin the BA and cell auxin NAA is the dominant factor induced proliferation rooting. Single-factor, two-factor and three-factor experimental results show that adding three hormones to induce the formation of tissue culture favorable, in particular: For the induction of the best medium MS 2,4-D0 .6 mg / L NAA0.4mg / L BA 1.0 mg / L sucrose 25g / L Joan powder 7g / L; plantlets following on behalf of the proliferation of the best medium MS 2,4-D0 .2 mg / L BA1.5mg NAA0.4mg / L / L sucrose 25g / L Joan powder 7g / L; rooting the best medium is MS 2,4-D0 .2 mg / L NAA0.4mg / L BA 1.0 mg / L sucrose 25g / L agar 7g / L; induced bulbs most the good hormone combination of MS 2,4-D0 .2 mg / L NAA0.4mg / L BA 0.5 mg / L sucrose 25g / L agar powder 7 g / L; pH 5.8 in medium, light 12h / d, light intensity 2000lx. From inoculation to transplanting, a cycle takes about three and a half months. Activated carbon added to the medium a positive role, but has little effect on proliferation, rooting and final seedling. Autumn leaves shamrock seedling time, reduce costs, nutrition rich matrix normal management can be transplanted to the hardening of tissue culture, transplant survival rate of up to 100% when the temperature 20 ~ 30 ℃. 3 autumn leaves shamrock maximum net photosynthetic rate in the summer, the average net photosynthetic rate is bigger in summer than in spring than autumn, the spring net photosynthetic rate most significant factor is the atmospheric temperature, the summer net photosynthetic rate mainly by intercellular CO2 concentration and the influence of water content, the fall of net photosynthetic rate by intercellular CO2 concentration and light intensity, three seasons of intercellular CO2 concentration and net photosynthetic rate negatively correlated. Spring light compensation point about 20μmol · m-2 · s-1, light saturation point is 1300μmol · m-2 · s-1; summer light compensation point about 45μmol · m-2 · s-1 light saturation The point is 900μmol · m-2 · s-1; autumn light compensation point, light saturation point is about 80μ · molm-2 · s-1 600μmol · m-2 · s-1. 4. Hongye Oxalis lower water use efficiency, drought capacity is relatively weak. Three seasons water use efficiency highest in summer, followed by spring and autumn the lowest water use efficiency is negative.
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