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This article as test material Hotan \; on this basis, further determined by HPLC granatum, pomegranate seeds and pomegranate leaves, rutin, quercetin, kaempferol pigment content, the distribution law of the understanding of flavonoids; analysis by orthogonal experimental ethanol concentration, extraction temperature, solid-liquid ratio, ultrasonic treatment time are four factors that impact on the extraction rate, in order to determine the optimum extraction parameters of pomegranate leaf flavonoids; same time, with diphenyl picrylhydrazyl (the DPPH) · Radical microplate method and lard anti-oxidation test to measure the strength of antioxidant activity of the pomegranate extract; finally through the tube dilution method, and a solid plate medium dilution method, flavonoids against Staphylococcus aureus, colon bacilli, antimicrobial effect, in order to provide a theoretical basis for the development and utilization of pomegranate resources in Xinjiang. The main conclusions are as follows: 1) qualitative test results show that: pomegranate seeds, peel and leaf ethanol extract redox reaction occurs, pink foam;, green and brown material generated during the reaction of the phenolic hydroxyl; complexation reaction, yellow-green fluorescence, these phenomena have pomegranate seeds, peel and leaves contain flavonoids. 2) quantitative test measurement results: Spectrophotometry HPLC method for the determination of pomegranate leaves, peel and pomegranate seeds total flavonoid content (the following results on a dry matter basis) granatum 5.804%, the pomegranate leaf 4.622%, the pomegranate seeds 1.056%; pomegranate seeds rutin content up to 1936.24 g / kg kaempferol content to 0.211 g / kg but does not contain quercetin, pomegranate peel and pomegranate leaves do not contain these three substances. 3) Ethanol extraction: tests showed that the highest flavonoids leaching rate of 70% aqueous ethanol, 60% ethanol and 70% ethanol flavonoids leaching rate difference is not significant, but significantly higher than 40%, 50%, 80%, 90% ethanol flavonoids leaching rate. 4) extraction temperature test: With the extraction temperature rise flavonoids leaching rate gradually increases, but at 80 ℃, flavonoids leaching rate declined. 5) extraction time trial: between 1-2.5 h, the extraction time, to extend the extraction time can increase the amount of pomegranate leaf flavonoids leaching flavonoids leaching rate; longer than 2.5 h, the flavonoids in pomegranate leaves to the solution The diffusion speed solution flavonoids balance, extend the extraction time to reach the rate of diffusion of the pomegranate leaf flavonoids leaching rate. 6) liquid ratio test showed that: increase the amount of solvent to increase the solvent flavonoids absolute ability to dissolve the solid to liquid ratio of 1:15 when flavonoids leaching rate and solid-liquid ratio of the flavonoid no significant differences in the amount of leaching 1:10. 7) ultrasonic treatment on the extraction rate of the pomegranate LEAVES great influence on the ultrasonic treatment for 0.5 h when the flavonoid content has reached 4.72%, under the same concentration of solvent and the extraction temperature, the ratio of liquid feed to an ultrasonic treatment to achieve the same The extraction rate savings of more than five times. 8) the pomegranate leaf flavonoids Orthogonal optimum extraction conditions: 60% ethanol, ultrasonic processing time 10 min, the liquid feed ratio was 15:1,70 ℃ reflux extraction. 9) vitro antibacterial test test results show that: pomegranate rind extract against Staphylococcus aureus, Escherichia coli have antibacterial activity. Extracts against Staphylococcus aureus minimum inhibitory concentration was 0.781 mg · the mL of -1 ; E. coli minimum inhibitory concentration of 3.125 mg · mL -1 ; Pomegranate leaf extract against S. aureus, E. coli also has antibacterial activity, but the minimum inhibitory concentration than granatum extract concentration were 12.5 mg · mL of -1 and 25 mg · mL -1 , the antibacterial effect a significant difference. 10) with diphenyl picrylhydrazyl (DPPH) · radicals the microplate reader law and lard antioxidant test determination pomegranate leaves and pomegranate peel extract antioxidant activity: pomegranate leaf extract of pomegranate rind on (DPPH) · radical scavenging rate of 93.35% and 80.65%, respectively; pomegranate leaf extract of pomegranate rind lard have good anti-oxidation.
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