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The Relationship between Arabidopsis Transcription Factor CBF1 and the Photoprotective Mechanism in Lycopersicon Esculentum Mill at Low Temperture under Low Irradiance

Author: ZhangYuJie
Tutor: BiYuPing
School: Shandong Normal University
Course: Botany
Keywords: CBF1 Tomato Plant Chilling Tolerance Photosynthetic capacity
CLC: Q943
Type: Master's thesis
Year: 2007
Downloads: 236
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Abstract

Damage caused by the low temperature on plant performance membrane lipid phase state change, the increase in the permeability of the membrane, increase the activity of the body's oxygen and free radical content, secondary metabolites accumulation. These phenomena are mainly due to low temperature on the destruction of the plant cell membrane systems, resulting in the various enzymes involved in the life activity structure is destroyed, the activity is decreased, so that the cell's metabolism system disorders. Photosynthetic system is also very sensitive to low temperature stress and massive accumulation of reactive oxygen species under low temperature stress conditions, the photosynthetic apparatus hurt by oxidative stress, light-harvesting capacity, photochemical efficiency are reduced to different extents, catalyzed photosynthetic dark reaction The activity was also reduced due to low temperature, the final performance of the photosynthetic rate reduction. In this paper, Arabidopsis (Arabidopsis thaliana) Columbia ecotype CBF1 (CRT / DRE bindingfactor1) gene for the purpose of the gene, the Arabidopsis the CBF1 gene plants expression vector pROKII, introduced into Agrobacterium application Agrobacterium-mediated leaf disc transformation 35S-CBF method into tomato (Lycopersicon esculentum Mill). Its overexpression in tomato transgenic plants. Its validation of molecular biology and photosynthetic physiological indicators of the test. The main results are as follows a low temperature under low light stress of transgenic plants PS II low-temperature low-light processing three days later, transferred Arabidopsis CBF1 gene-tomato (ACBF1-tomato) Fv / Fm, apparent photosynthetic quantum transfer efficiency (ΦPS Ⅱ) and photosynthetic electron transport quantum efficiency (ETR), although has declined, but compared with the wild control the degree of inhibition have been greatly improved. Transgenic lines of non-photochemical quenching (NPQ) than wild control, indicating that inhibition of photosynthetic carbon assimilation in the case of low temperature and low light stress, transgenic tomato leaves NPQ better able to play the dissipation of excess excitation energy, protect the light The role of the system. Chilling under low light stress on the impact of ACBF the PS Ⅰ's transgenic plants tomato oxidation state P700 was significantly higher than that of wild control, the CBF pathway plants PS I also have a protective effect. 3 chlorophyll content of low temperature and poor light will also affect chlorophyll biosynthesis, the destruction of the structure of the chloroplast. Low Light found that the overall trend is in the control of chlorophyll a, chlorophyll b, chlorophyll ab total content were significantly lower than the transgenic lines, and chlorophyll a / b ratio, higher than the transgenic plants, description transgenic plants better low temperature and low light resistance. 4 low-temperature low-light stress SOD transgenic plants the activity ACBF1 tomato SOD enzyme vitality improved to a certain extent to improve the ability of tomato resistance to low temperature and poor light. 5 of malondialdehyde (MDA) content: MDA may well reflect the degree of oxidation of membrane lipids under chilling injury. Low Light control MDA was significantly higher than the transgenic lines. 6 soluble sugar content: low-temperature low-light stress in transgenic plants was significantly higher content of soluble sugar, and higher than the wild-type. The above results show that the same Low Light, for the wild-type plants, has caused obvious symptoms of stress; the transgenic seedlings due to the presence of the body Arabidopsis CBF1 reduce the effects of low temperature and poor light stress. Thus, we can determine, of Arabidopsis CBF1 the constitutive expression does improve tomato resistance to low temperature and poor light resistance.

The Relationship between Arabidopsis Transcription Factor CBF1 and the Photoprotective Mechanism in Lycopersicon Esculentum Mill at Low Temperture under Low Irradiance

Abstract

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