|
This test first to red xanthina chromosome count , and its karyotype analysis. The results showed that red xanthina chromosome number of 21 , the karyotype formula is 2n = 3x = 21 = 18m 3sm, belongs to 2A type . Test and two different DNA extraction methods comparison screened CTAB ball method generally suitable for rose plants ; and red xanthina genomic DNA as template optimized SSR reaction system and determine the reaction conditions , from 88 pairs of SSR primers screened more normality 10 pairs of primers , polymorphism percentage reached 47.62% ; through the red thorn rose , rose , etc. 32 varieties of SSR analysis, clustering results are divided into four categories: Rosa and four red roses clustered together , Rose carpets and hula girl together as a class, Snow , South Korea, Sunday is the 1st and the Royal Ba Xinuo clustered into one group , another variety of rose cultivars were clustered into one class . Also targeted for the red xanthina improved varieties breeding and horticultural breeding strategy formulation , to carry out cross-breeding work , verify using SSR markers assisted selection hybrid parents the feasibility and effectiveness . In F1 hybrid breeding , through plant performance traits and adaptability of observation, elect eight meritorious plants ; and after cold SSR markers on the 1st and red brocade Rosa 's five offspring per plant for real identification , clustering result is mixed with 5-2 and 5-4 hetero male together as a group, miscellaneous 5-1 , 5-3 and miscellaneous miscellaneous 5-6 and female together as a group, and morphological identification results similar .
|