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Research of Germplasm Resources and Genetic Diversity of Snakegourd

Author: QianChunLu
Tutor: ZhangMingFang
School: Zhejiang University
Course: Olericulture
Keywords: Trichosanthes Floor Cultivar identification Genetic Diversity Kinship AFLP markers SDS-PAGE
CLC: S567.239
Type: Master's thesis
Year: 2007
Downloads: 274
Quote: 2
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Abstract

Kirilowii (Trichosanthes kirilowii Maxim) is a perennial twining vine the Cucurbitaceae kirilowii genus, is a traditional Chinese herbal medicines. This study investigated the 12-Trichosanthes floor materials trait characteristics, the ploidy of the material is determined by cell staining tablet, at the same time with the AFLP molecular marker analysis and evaluation of the genetic diversity and phylogenetic relationship of 12 copies of the material, and finally with The method of SDS-PAGE analysis of various materials, seed storage protein, to lay the foundation for future use and medicinal ingredients kirilowii analysis. The experimental results show that, the the 12 parts China Trichobitacin floor material outside or differences in traits. The experimental materials used three diploid, have 9 Octoploid. AFLP molecular marker on the 12 Trichosanthes floor material detected an average of 97.36% of the polymorphism. This experiment using 14 pairs of AFLP primers amplified a total of 628 polymorphic bands per pair primers 44.86 polymorphic bands. Statistics AFLP bands to the presence or absence of the bands principle, obtain the needed 0-1 matrix, the similarity between the two varieties of type Jaccard and Nei and Li similarity coefficient expression. Establish Jaccard and Nei and Li similarity coefficient matrix, and on the basis of the Jaccard similarity coefficient matrix, NTSYS-PC software the SAHN clustering analysis, the 12 the Trichosanthes floor material of genetic diversity and phylogenetic relationship with the UPGMA algorithm dendrogram . AMOVA analysis (analysis of molecular variance) estimated the genetic variation in the phylogenetic tree divided group and between groups with Arlequin software. The statistical results show that both Jaccard and Nei and Li similarity coefficient values ??(Jaccard similarity coefficient distribution at 0.0645 to .7270, Nei and Li similarity coefficient distribution at .3252 to .8678), but reveal the same genetic relationship. Jaccard similarity coefficient matrix clustering 12 of Trichosanthes floor materials phylogenetic relationship tree, all the materials can be clustered into two groups, in diploid material (1,2,3) Group 1 The remaining the octoploid material for the second group. Group differences obvious by the group branch (group 1 No. 2 materials branch and the 6th group material branch). AMOVA analysis of genetic differences between the results, diploid and octoploid for 99.67% of the total variation, variation within groups accounted for only 0.33% of the total variance. Kinship tree to reveal the genetic relationship of various of Trichosanthes floor types and no significant correlation and geographic distribution. All materials, the smallest genetic distance from the Henan Jiaozuo Huai drug producing areas 11 and 12 materials, may be due to the geographic distribution to smaller isolation. The cluster analysis showed that each the Trichosanthes floor material with heterogeneity and genetic similarity. As a traditional Chinese medicine, different the Trichosanthes floor type have different medicinal ingredients. Experimental material, the No. 8 material from Anyang, Henan is famous for its tuberous roots from Shandong Changqing No. 6 material known for its fruit, these two materials are best recognized the medicinal effects of traditional Chinese medicine profession the Trichosanthes floor types. In the second group, on the 6th of material as the value of the drugs used melon material separate branch to separate and the other medicinal kirilowii area, which reveal the genotype of the specific material has high medicinal value. Coincidence that we found from the 10th Jiaozuo, Henan materials from the No. 6 Shandong kirilowii and No. 8 Anyang pollen genetic distance Trichosanthes floor material type (from the material genetic distance 0.16 from the No. 8 material genetic distance for 0.14 ), which implies The has the wild kirilowii in closest Anyang the pollen and Shandong kirilowii genotype, which may have higher medicinal value. The Trichosanthes floor is typical of dioecious plants, it is the ideal material to study male and female differences, try this experiment highly polymorphic AFLP molecular markers to identify the differences the Trichosanthes floor intersexuality (experimental results) finally found more difficult. Seed storage protein analysis showed that each kirilowii material type seed protein differences, and the differences in the genetic relationship between various materials. In this experiment, we draw the following conclusions: (1) AFLP molecular marker technology can accurate identification of separate morphological complexity of similar the different genotypes Trichobitacin Floor type, which is very effective in China of Trichosanthes kirilowii germplasm resources between genetic diversity evaluation . High levels of genetic diversity and variation level (2) China of Trichosanthes kirilowii germplasm resources, more than 90%. (3) kirilowii diploid group and the difference between the octoploid group is far greater than the differences within the group. (4) No. 6 materials from Shandong Changqing as the medicinal value of the highest the Trichosanthes floor if material is octoploid the medicinal materials genotype is the most specific type of Trichosanthes floor. (5) Trichosanthes Floor type seed storage protein contained differences, and the genetic differences are obvious differences on the DNA level.

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