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Yuan Hu (Rhizoma Coridalis) and Zhejiang Fritillaria (Bulbus Fritillariae Thunbergii) is produced in Zhejiang Province, the famous herbs \Pan'an because of its unique geographical environment and climatic conditions, so that the production of these two herbal quality excellent, stable texture, has formed a certain scale of production base. However, the herbal from planting base, a large number of raw herbs still acquisition in loose farmers, non-standard production conditions lead to uneven quality varieties, there may be a considerable degree of confounding, which requires the establishment of a standard fingerprint geoherbs maps to guide GAP standardized production. For this purpose, by high-performance liquid (HPLC) chromatography technology and Fourier transform infrared (FTIR) spectroscopy, Zhejiang Province produced authentic Yuan Hu and Zhejiang Fritillaria medicinal herbs and other national origin produced by the analysis and comparison of the fingerprints of comprehensive chemical fingerprint of Zhejiang production Yuan Hu, geoherbs, Zhejiang Fritillaria. 1. Yuan Hu, the chemical fingerprint of Corydalis Corydalis (Corydalis yanhusuo W.T.Wang ex Z.Y.Su et C.Y.Wu) for CORYDALIS plants dry tubers, this paper the tetrahydropalmatine standard products for the control, The effects of the different samples of different extraction methods and HPLC process flow, and by the addition of small molecule organic acids and bases (acetate, triethylamine), optimization of the mobile phase system, to improve the chromatographic peak peak shape and separation After trial and error to determine the mobile phase gradient, the medicinal ingredients made good separation on the basis of stable, after the preparation, analysis, calibration, Pan Yuan Hu HPLC fingerprint and compares the different processing method, the differences between the different origin produced Yuan Hu. The results show that: corydalis on fingerprints that reflect the similarity between herbs corydalis by the same method in different regions in the province produced processing treatment HPLC fingerprint was no significant difference, but production Yuan Hu and other provinces Zhejiang Corydalis exist some differences determined by comparing the feature regions of the Pan Yuan Hu: 9min 45min, Zhejiang Corydalis small peak, Jiangsu and Shaanxi samples, but not obvious or no; 70min or so, the two provinces of Corydalis There is a small peak, Zhejiang Corydalis but no produced Yuan Hu, geoherbs, identify Pan'an provides a scientific basis. The paper compares different processing methods on the market Lantern Hu, results showed that: the sulfur fumigation Yuan Hu HPLC spectra peaks reduce the number may be some component changes or loss, is not recommended to use; unprocessed raw herbs and herbs cooked by boiling water in the chemical composition on the occurrence of a significant change from the HPLC fingerprint can directly identify through cluster analysis and principal component analysis method can well distinguish three different processing methods processing element Hu. Choose a typical HPLC chromatogram according to the results of the above analysis, a total of of Yuan Hu HPLC fingerprint mode, and gives the fluctuation range of the peaks of the relative retention times and relative peak area reserved. In addition to establishing the HPLC fingerprint FTIR spectra of different origin Yuan Hu, and similarity analysis, the results show that the similarity of different origin Yuan Hu Basic 0.90 or more, it is difficult to distinguish, but the processing of the different processing methods The FTIR spectrum Hu similarity there are significant differences can be distinguished by this method. 2. The establishment of the chemical fingerprint of Zhejiang Fritillaria Fritillaria thunbergii Liliaceae Zhejiang Fritillaria (Fritillaria thunbergii Miq.) Dry bulb. Is mainly produced in Zhejiang, Jiangsu, Anhui and other places, the main component of alkaloids. Different Fritillaria vary alkaloid types contained in this article is a little change, according to the Chinese Pharmacopoeia (2005 edition a) Zhejiang Fritillaria alkaloids extracted instead of using ultrasonic extraction heated to reflux prepared for the test sample, examines the different mobile phase, through trial and error to determine the gradient of the mobile phase. Since most of the alkaloids in Fritillaria in UV absorption, evaporative light scattering (ELSD) detection instead of UV detection, 5 Pan Zhe Bei, a Fritillaria, Ningbo, Jiangsu Province, a production Zhejiang Tony the fritillary, Anhui scallops Fritillary HPLC-ELSD profiles, by comparison found that there are obvious differences between the East Bay, Anhui shellfish, fritillary, Fritillaria determine specific peak of Anhui Tony: No. 6, 12, 13 No. 15 peak; East Tony the Fritillaria variants, but can be seen from the HPLC chromatogram significant difference between the two East Tony peaks were identified: No. 2, No. 7, No. 9, No. 18 peak ; 2 Chuanbei samples for different growth years of the same kind of fritillary bulbs growing years longer the fritillary and Zhejiang Tony differences obviously, the growth years shorter Chuanbei Zhejiang Tony similar to its secondary metabolites product accumulation is a dynamic process of change; Fritillaria from Pan'an and Ningbo were no significant differences between the cluster analysis and principal component analysis are distributed together, but from Nantong, Jiangsu Zhejiang Tony Zhejiang produced Zhejiang Tony in the the dendrogram separation, may be due to different cultivation environment that paan production the Zhejiang Tony genuineness. At the same time, the study by FTIR technique to obtain infrared spectra of the different species of Fritillaria map through the effective fingerprint information extracted spectra using multivariate statistical clustering analysis based on chemometrics found with HPLC clustering Figure results in a large pattern of consistent peace Tony can not distinguish between the East Bay in the HPLC dendrogram the FTIR clustering spectra but can be distinguished, but needs further study whether the FTIR method is able to identify Fritillaria.
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