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Effects of Cadmium on Peanut Qualify, Physiological Response and Type Ⅱ Metallothionein Gene Expression

Author: YangZhiYi
Tutor: DanShiHua
School: Ocean University of China
Course: Of Food Science
Keywords: Peanut Cadmium Physiological mechanisms Metallothionein gene
CLC: S565.2
Type: Master's thesis
Year: 2010
Downloads: 83
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Abstract

The cadmium abiotic must element, is also one of the most harmful heavy metal environmental pollution, the main source of naturally occurring and man-made pollution, such as mineral exploitation, industrial production in the \Through the food chain, it can be absorbed by the body, and the accumulation in the liver, kidney, pancreas, thyroid and bone. Will affect their health status when the cadmium absorbed by the body reaches a certain dose, can cause a variety of serious diseases, such as hypertension, bone pain, disease, kidney disorders, liver damage, pulmonary edema, and anemia. The peanut is both the world's major oil crops, important sources of vegetable fats and oils and food processing raw materials. Of both the world and peanut-producing countries, is also the largest exporter. In recent years, China's exports of peanuts and peanut products, heavy metals cadmium exceeded the growing phenomenon of affect the peanuts food security. Therefore, how to effectively control the peanut kernels Cd pollution has become a research hotspot. In this thesis the soil of potted plants and nutrient solution culture combined to study the quality and impact of physiological and biochemical mechanisms of cadmium stress on different peanut varieties, as well as peanut seedling metallothionein gene expression with different cadmium treatment level. The results are as follows: (1) cadmium treatment differences impact on the yield and quality of peanut genotypes. The cadmium treatment level of 12mg/kg, peanut seed cadmium content in the control group (CK) There are significant differences, and the presence of genotypes between cadmium accumulation significant difference, filter out the variety the XD011 and high accumulation of low accumulation of cadmium in the tested varieties cadmium varieties XA004. Of different genotypes peanut protein content of the rate of change in the treatment of cadmium exists a difference between the rate of change of -12.3% -13.9%. Cadmium on peanut pod yield and fatty acid content is not significant, but all of the Cd Peanut Seeds cadmium content have been exceeded, can not be directly edible. (2) the impact of cadmium stress on peanut different growth stages physiological mechanisms there are differences, and pod period most remarkable and significant differences between varieties. Peanut harvest time, little the Cadmium Stress pod yield change, but significantly reduced the shelling percentage and kernel yield; the cadmium stress leaves major physiological changes in indicators of principal component analysis, drawn MDA content, POD vitality and total chlorophyll content a major impact factors, these three indicators can be used as a sensitive indicator of cadmium, as well as peanut cadmium pollution stress diagnostic indicators. By cadmium stress indicators and yield of the first component the change cluster analysis obtained in the tested varieties Huayu 22, Huayu 23 and Luhua 14 relative to the abundance of flowers, Huayu 20 and Luhua 12 for cadmium-insensitive varieties. (3) Cadmium in peanut vivo distribution characteristics are as follows: to cadmium treatment level, shank gt; roots gt; stem gt; leaves gt; seeds; to cadmium treatment level 3mg/kg, stems gt; roots gt; Ye gt; seeds gt; handle; When Cd level reached 12mg/kg, root gt; leaves gt; stem gt; seeds gt; handles; cadmium the treatment concentration mention to 24mg/kg leaves gt; stem gt; root gt; handle of gt; seeds. With increasing concentration of cadmium pollution of cadmium in the peanut crop gradually accumulated to leaf stem, rather than the root is the most important parts of the accumulated. (4) Construction of a recombinant plasmid PMD18T-AhMT2 recombinant plasmid copy number and Cp values ??has a good linear relationship, and the melting curve peak single, mixed peaks, can effectively exclude non-specific signal interference, successfully established accurately analysis peanut AhMT2 mRNA expression levels SYBR Green Ⅰ real-time fluorescence quantitative PCR experiments. Observed and analyzed using the method of Cadmium on peanut seedlings of different vegetative organs AhMT2 mRNA expression level differences, confirmed the Cd levels with peanut AhMT2 mRNA expression has some correlation, correlation coefficient R2 greater than 0.9.

Effects of Cadmium on Peanut Qualify, Physiological Response and Type Ⅱ Metallothionein Gene Expression

Abstract

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