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Tested strains of tomato Fusarium wilt and to Chinese herbal F, through the liquid - liquid partition, column chromatography isolated from the Chinese herbal F major antibacterial active ingredient 2006-HS, the main findings are as follows: 95% ethanol as the extraction solvent, using cold soak extraction. In vitro experiments showed that the crude extract of tomato Fusarium wilt with strong activity. Crude extract of tomato wilt pathogen 3d and 4d virulence regression equation were y 3 = 0.8516x 3.8502 (r = 0.9697) and the y 4 = 0.7103x 4.0667 ( r = 0.9557), and calculate the 3d and 4d EC 50 respectively, 0.224g / L and 0.206 g / L. 2 petroleum ether / 70% ethanol (V: V = 1:3) to the crude extract was subjected to liquid - liquid separation, the petroleum ether to except for a part of the pigment and impurities, and the water phase of the polarity of the material in crude extracts separated out, the active substance content in the resulting oil was increased, the inhibition increased to Fusarium wilt of tomato. Oil relative tomato Fusarium wilt away from the body test 3d and 4d virulence regression equation y 3 = 1.0274x 3.7194 (r = 0.9766) and y 4 = 1.0082x 3.7751 (r = 0.9879), of tomato Fusarium 3d and 4d, EC 50 0.176g / L and 0.164 g of / L, compared with the crude extract, 3d of EC 50 value decreased 21.4%, 4d of EC 50 value dropped by 20.3%, significantly enhanced antibacterial activity. 3 using the silica gel column, eluent petroleum ether and ethyl acetate, and twice by column chromatography, to obtain the crystal. The crystals of the secondary column chromatography the Fusarium pathogen 3d and 4d virulence regression equation y 3 = 0.8843x 4.5025 (r = 0.9481) and y 4 = 0.9357x 4.3982 (r = 0.9647); the 5d virulence regression equation for y 5 = 0.9651x 4.3309 (r = 0.9612). Crystal tomato Fusarium 3d, 4d and 5d the EC 50 were 0.037g / L, 0.044g / L and 0.049 g / L, with time, the activity declined, EC < sub> 50 value increases slightly. Compared with the crude extract 3d EC 50 value dropped by 79.0%, the the 4d EC 50 15 H 16 O 3 . 6 Infrared spectroscopy active substance 2006-HS The molecular structural formula containing a C = O group, a benzene ring, a methyl group. Combining the NMR data, analyze the identified active substance 2006-HS chemical structural formula.
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