Dissertation > Excellent graduate degree dissertation topics show

Study on Analysis of Pharmaceutical and Pesticide Residue Using Chemiluminescence

Author: XiaoNa
Tutor: ChenChao;CuiYaLi
School: Northwestern University
Course: Biochemistry and Molecular Biology
Keywords: Chemiluminescence analysis Flow-injection analysis Hydrochloride adrenaline Metaraminol bitartrate Organophosphate pesticides
CLC: S481.8
Type: Master's thesis
Year: 2007
Downloads: 275
Quote: 1
Read: Download Dissertation

Abstract


Chemiluminescence （CL） analysis, based on the emission during some chemical reactions, is a technique for the determination of trace analyte. Due to its high sensitivity, wide linear range and easy implementation, chemiluminescence analysis has been widely applied in pharmaceutical analysis, food safety, environmental science and life science etc. In this thesis three different CL systems, luminol-KIO4 CL system, luminol-KMnO4 CL system, alkaline luminol CL system, were introduced to detect some pharmaceuticals and the residue of pesticides using a flow injection chemiluminescence （FI-CL） method. The main content is as following:1. A simple chemiluminescence method for the determination of hydrochloride adrenaline with flow injection chemiluminescence （FI-CL） technique was proposed, which was based on hydrochloride adrenaline enhancing the chemiluminescence from potassium periodate and luminol in alkaline medimum. The linear range of the calibration curve was 1.0×10-8～3.0×10-6g/mL, and the detection limit was 1.5×10-9g/mL. The relative standard deviation was 1.3％(n=11, c=5×10-7g/mL,). The method has been applied to the determination of hydrochloride adrenaline in pharmaceutical samples.2. It was found that the chemiluminescence arising from the reaction of luminol with potassium permanganate in alkaline solution could be strongly enhanced by metaraminol bitartrate. Based on these observations, a new flow injection chemiluminescence （FI-CL） method for the determination of metaraminol bitartrate was proposed. The linear relationship between the intensity of chemiluminescence and the concentration of metaraminol bitartrate was in the range of 1.0×10-8～1.0×10-5g/mL with a detection limit of 3.7×10-9g/mL and the relative standard deviation was 2.5％(n=11, c=1.0×10-7g/mL). The method was successfully applied to the determination of metaraminol bitartrate in its pharmaceutical formulations. Moreover the reaction mechanism of the chemiluminescence system has been discussed based up on the fluorescent spectra, UV-visible spectra and some other experiments.3. A flow-injection chemiluninenscence （FI-CL） method was set up for determination of organophosphate pesticides in vegetables. The degradation of organophosphate pesticides can be achieved by photo-catalysis in K2S2O8 solution. The inorganic phosphorus （the products of degradation of organophosphate pesticides） could form vanadomolybdophosphoric heteropoly acid with the addition of vanadate and molybdaenate in sulfuric acid medium, which could oxidize luminol to produce CL emission. The CL emission intensity was linearly correlated with the phosphorus concentration in the range 1.0×10-9～3.0×10-6g/mL with a detection limit of 1.0×10-10g/mL. The relative standard deviation （n=11） was 2.1％for 2.0×10-7g/mL phosphorus. The recoveries ranged from 89.5％to 114％by adding 3 different organophosphate pesticides with amount of 1.00mg, 2.00mg pesticide per kilogram vegetable respectively. The method has been applied satisfactorily to the determination of organophosphate pesticides on mini chemiluminescence instrument.

Related Dissertations

  1. Study on Deleterious Substances in Coptis, Angelica and Mechanism of Alkaloids in Coptis to Cure Ⅱ Diabetes,R284
  2. Flow Injection Online Coupled with Atomic Absorption Spectrometry,O657.3
  3. Determination of Trace Arsenic in Environmental Samples with Gas-Phase Chemiluminescence Detection Following Flow Injection Hydride Generation,O657.3
  4. Developlment of Nanoparticle Mimic Enzyme-based Analytical Methods and Applications,O629.8
  5. Development and Application of the Analytical Technique and Shipboard Instrumentation for the Determination of Ultra-trace Soluble Reactive Phosphorus in Seawater,P734
  6. Study on the Determination Techniques for Ultra-trace Orthophosphate in Seawater,X834
  7. Determination of COD Based on the Electrode of a Boron-doped Diamond （BDD） Film and Fabrication of TiO2-BDD Heterojunction Photocatalysts,X832
  8. Study on the Fluorimetric Determination of Formaldehyde in Drink and Food Products by Flow Injection Analysis,O657.3
  9. Study on Flow-injection Chemilluminescence with Potential Adjustment Technique and Its Analytical Application,O657.3
  10. The Analysis, Determination of Polar Component with No Ultraviolet Activity and Its Application in Pharmaceutical Analysis,R917
  11. Ion Interference and Their Reduction Method in the Flow Injection Analysis of Chemical Oxygen Demand (COD) Using Boron-Doped Diamond (BDD) Electrode,X832
  12. Studies on Multiwalled Carbon Nanotubes Modified Electrodes and Their Application in Pesticide Determination,TQ450.263
  13. Studies on Nano-modified Amperometric Enzyme Biosensor and Its Application in Pesticide Residue Analysis,X839.2
  14. Isolation and Characterization of Organophosphate Pesticides Degrading Strains, Cloning and Expression of Ophc2 Gene,X172
  15. Investigation on the Post-chemiluminescence Analysis Using Potassium Permanganate as the Oxidant,O657.3
  16. Chemiluminescence chemiluminescence new system and new methods of research,O657.3
  17. Investigation on the Galvanic Cell Generated Electrochemiluminescence and Its Analysis Application,O657.3
  18. Investigation on the Microfluidic Chemiluminescence Biosensor Chip and the Chemiluminescence System,TP212
  19. Study and Application of the Flow Injection Biamperometry for Irreversible Redox Couple System,O657.1
  20. The Study of High Performance Liquid Chromatography Based on Chemiluminescence Detection,O657.3

CLC: > Agricultural Sciences > Plant Protection > Pesticide ( chemical control ) > Plant chemical protection theory > Pesticide residues
© 2012 www.DissertationTopic.Net  Mobile