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Tripterygium (Tripterygium wilfordii Hook F.) Department of Tripterygium Celastraceae woody vine, is a traditional Chinese herbal medicine Tripterygium the active ingredients contained in many kinds of content is low, but the activity was significantly diterpenoid alkaloids and obvious physiological activity of triterpenoids and other ingredients with anti-inflammatory, immunosuppressive, anti-tumor, anti-fertility. The plant Tripterygium resources chemical composition and preparation of the active ingredient, pharmacology and toxicology role, clinical applications to do a literature review. Tripterygium as a traditional Chinese herbal medicine, which drug agents for the treatment of rheumatoid arthritis, the treatment effect is significant. Triptolide strong activity, is commonly used as a preparation of Tripterygium class quality indicators and limited index components. Tripterygium preparations, however, is more than the crude extract, the chemical composition is very complex, simply to a particular compound can not be a comprehensive description of its biological activity, single component as a quality control indicators clearly unscientific. So, find of Tripterygium herbs and their preparations scientific and effective quality evaluation method is the Tripterygium study need to solve the problem. To this end, the experiment carried out research work of Tripterygium effective component of the group's preparation and quality analysis. This study: (1) the use of animal test methods, compare Tripterygium different extracts of herbs (peeled xylem) and its segmented parts of the immune inhibition activity; (2) the use of different chromatographic packings (positive phase and inverting), the parts of the pairs of the immune inhibition activity preferably tripterygium alcohol extract Its chloroform Preparation of the size of the effective component of the different active group, and their detection by TLC, HPLC and UPLC technology; (3) the use of HPLC and UV Tripterygium alcohol extract different segments A prime diterpene lactone, total alkaloid content was detected; (4) the use of modern separation techniques (pressure, HPLC preparation, etc.) and modern spectroscopic techniques, Zhejiang Province Tripterygium herbs collected from ethyl acetate fraction of the ethanol extract separation, purification and structure identification of compounds. The results showed: 1,95% alcohol extract the chloroform site points and ethyl acetate parts generate sub-hemolysin antibodies in mice inhibit its immunosuppressive activity. MDS-inverting resin stationary phase methanol - water system chloroform parts piecewise better determine triptolide ethanol extract parts of chloroform the best segmentation separation of effective components group conditions. A prime diterpene lactone, total alkaloid content were detected there are obvious differences in different parts of the content, including higher levels of chloroform parts of ethyl acetate extract. 4, at the same time, the use of UPLC fingerprint chloroform segment three component groups were analyzed to identify which part of the diterpene lactones and sesquiterpene alkaloids in peaks and higher levels of the component type, which diterpenoid lactones and alkaloids in higher levels. 5 points seven compounds were: 2 diterpenoids: Ray the phenolic terpenes ether (triptonoterpene methy lether, cp1) and Ray phenol lactone (triptophenolide, cp4); sesquiterpene alkaloid components: Thunder God Fujiyoshi of alkali (Wilforgine, cp5) and Tripterygium of alkali (Wilforine, cp6); Triterpenoids: the any card lacquer -5 - ene-3β, 28 - diol (of Glut-5-en-3β, 28-diol 2 sterols, cp3);,: β-sitosterol (β-Sitosterol, cp2), and carrots of glycosides (daucosterol cp7,). Wherein, cpl, cp4, cp5, cp6 the reported active compound. The experiment through different active component of Tripterygium group preparation process research and combined with the Tripterygium anti-inflammatory immune the pharmacodynamic activity screening results, to establish a qualitative analysis of the determination of multiple anti-inflammatory immune the UPLC content of the active ingredient and fingerprint combination Tripterygium herbal quality evaluation methods, both from content limits and the overall chemical characteristics, comprehensive scientific assessment and effective control of the intrinsic quality of Tripterygium herbs and their preparations, and to lay the foundation for the sustainable development of Chinese of Tripterygium medicinal resources.
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