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The application of modern analytical techniques in the study of zolmitriptan

Author: Shi
Tutor: ShiJie；WuYongJun
School: Zhengzhou University
Course: Drug analysis
Keywords: Zolmitriptan HPLC Chemiluminescence Ion Selective Electrode Analysis Methodology
CLC: R917
Type: Master's thesis
Year: 2007
Downloads: 92
Quote: 0
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Abstract


Pharmaceutical analysis is the idiographic application of Analytical chemistry technology in Pharmaceutical field and a scientific method of research and development of overall medicine quality control. The advancement of Pharmaceutical analysis, especially the progress of instrument analysis and computer technology, makes a solid foundation for Pharmaceutical analysis. The purpose of Pharmaceutical analysis is in various fields of Pharmaceutical Sciences to detect, identify and determine certain components in samples using all kinds of analytical methods and meeting demand of different purposes and requirements.This Article takes advantage of many a pharmaceutical analysis method to determine medical components in different carriers of Zolmitriptan, a new medicine. It also makes compare and profound discussion to priorities of all methods and speciation.This Article is composed of two parts: the preface briefly introduces the current progress of Zolmitriptan; the test part turns to all analytical methods, their research production, methodology comparison and speciation, as follows:1、Determination of Zolmitriptan tablets by HPLCTo establish a method for the content determination of Zolmitriptan tablets by HPLC. Using a Kromasil ODS-3 C18 column （250 mm×4.6 mm,5μm） with the mobile phase of 0.05% triethylamine in water （adjusting to pH 2.75 with phosphoric acid） and acetonitrile （90:10）. Fluorescence detection was performed at 225 nm （excitation） and 360 nm （emission）. The calibration curve was linear within a range of 1～80μg·ml-1（r = 0.9998）. The assay method is accurate and simple with high recovery.2、HPLC determination of Zolmitriptan in human plasmaA method for determination of Zolmitriptan in human plasma by HPLC was developed. Using a Kromasil ODS-3 C18 column （250 mm×4.6 mm,5μm） with the mobile phase of 0.05% triethylamine in water （adjusting to pH 2.75 with phosphoric acid） and acetonitrile （90:10）. Fluorescence detection was performed at 225 nm （excitation） and 360 nm （emission）. The calibration curve was linear within a range of 2～200ng·ml-1（r = 0.9993）. The assay method is accurate and simple with high recovery.3、Chemiluminescence determination of Zolmitriptan by flow injection analysisA new flow injection procedure was developed for the determination of Zolmitriptan based on the inhibition of the chemiluminescence from Luminal-K3[Fe（CN）6]-NaOH system. The linear range for Zolmitriptan was 2.0×10-6～1.2×10-4g·ml-1and the limit of detection was 7.6×10-7g·ml-1. The relative standard deviation （n = 11） was 1.2% for 2.5×10-5 g·ml-1 Zolmitriptan. This method was applied to the determination of Zolmitriptan in tablets with satisfactory results.4、Chemiluminescence determination of Zolmitriptan drugs in KMnO4-LminolsystemsA new method of chemiluminescence determination of Zolmitriptan drugs in Luminal-KMnO4 systems was established. It showed that Zolmitriptan could potently inhibit chemiluminescence of Luminal-KMnO4 systems, based on which the determination of Zolmitriptan drugs tested by flow-injection analysis with chemiluminescence was described. Zolmitriptan linearly ranged from 1.0×10-6 to 6.0×10-5g·ml-1 and the limit of detection was 8.4×10-8g·ml-1. The relative standard deviation （n = 11） was 1.4% for 2.5×10-5g·ml-1. Thanks to its simplicity, efficiency and high sensitivity, the method could be applied to the determination of Zolmitriptan in tablets. Furthermore the mechanism of the reaction was also expounded in this paper.5、Determination of Zolmitriptan tablets by ion selective electrodeThe influences of various ionic complexes, concentration of activator inmembrane phase and plasticizer on the properties of electrode were studied byorthogonal design. A PVC membrane electrode of graphite inner-conductivenorfloxacin which had good stability and reproducibility was prepared. The electrode response ranged between 5×10-5～5×10-3 mol·L-1 with a slope of 57.8 mV/pC. The detection limit was 2.3×10-5mol·L-1. The method can be used for determination of the concentration of metoprolol in tablets simply, rapidly and accurately.

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