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Iron and phosphorus is an essential element for plant . Studies have shown that low phosphorus can promote plant iron absorption and effective iron content of the plants , however, the interaction of the iron / phosphorus physiological and molecular mechanisms of very little information , especially in rice . Our previous studies have shown that when naat1 be able to reply to Fe ( III ) to Fe ( III ) as the sole source of iron and phosphorus deficiency Fe ( III ) - P ) under the conditions of absorption mutants defective in Fe ( III ) source of iron Fe ( III phenotype ) P ) , but the exact mechanism remains unclear. Mutant mutant naat1 with ethyl methanesulfonate (EMS) in the Fe ( III ) - p under the conditions of the filters can not reply to the phenotype of the material . Screening to a root system back to normal , shoot can not be reused the green double mutant , named naat1/M13 . The mutant , 10 days after germination in the Fe ( III ) P conditions , be able to reply to the naat1 root phenotype compared with the wild type still showed yellowing symptoms . In addition, when Fe (Ⅱ) as the sole source of iron conditions , 10 days after germination , also showed symptoms of yellowing . The root of the real-time quantitative PCR ( qRT - PCR ) analysis showed that in the Fe ( III ) P conditions , naat1/M13 compared with naat1 , iron deficiency response genes (OsIRO2, OsNRAMP1, OsYSL2, OsYSL15, OsIRT1, OsIRT2, OsNAS1 OsNAAT1 ) downregulated ; under the Fe ( III ) - P conditions , naat1/M13 compared with naat1 , OsIRT1 with OsIRT2 expression down , but OsIRO2 , OsNRAMP1 OsYSL2 OsYSL15 OsNAS1 OsNAAT1 no significant differences ; conditions of Fe ( II ) P The next naat1/M13 compared with naat1 iron deficiency response genes were down . The shoot qRT-PCR analysis showed that the Fe ( III ) P conditions , naat1/M13 compared with naat1 , iron deficiency response related genes were down-regulated ; Fe ( III ) - P conditions , naat1/M13 compared with naat1 , OsIRO2, OsNRAMP1, OsYSL15, OsIRT2, OsNAS1 and expression of OsNAAT1 down , but there was no significant difference OsIRT1 , OsYSL2 upregulation ; Fe ( II ) P conditions , naat1/M13 compared with naat1 , iron deficiency response gene expression were no significant differences . Genetic analysis showed that the M13 by a single recessive gene control . Gene mapping to the first two STS ( SEQUENCE specific site ) mark ( S1 - 8989 and S1 - 9038 ) on chromosome map-based cloning method , the physical distance of approximately : 49kbp . According to the forecast of the TIGR this interval 8 encoding gene . Our results show that M13 involved in iron / phosphorus interactions in plants .
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