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Objective To investigate VEGF, COX-2 in chronic cervicitis, cervical intraepithelial neoplasia-like change, the relevance and significance of cervical carcinoma and CD31 markers neovascularization and pathological analysis. The method is two-step application of immunohistochemistry to detect 47 cases of cervical cancer and 17 cases of cervical intraepithelial neoplasia-like change of VEGF in the tissues and 15 cases of chronic cervicitis tissues, the expression of COX-2, CD31. Results 1. VEGF in chronic cervicitis, cervical intraepithelial neoplasia samples and Cervical group expression rates were 6.0%, 23.5%, 55.3%, and the three groups, the difference was significant (P <0.01). Cervical intraepithelial tumor-like change (CIN) group, the positive expression rate, CIN Ⅰ grade CIN Ⅱ level and CIN Ⅲ level to 12.5%, 25.0%, 40.0%, three groups differences without significantly with sex (P> 0.05). The VEGF cervical cancer patients age, histological type unrelated (P> 0.05), clinical stage, lymph node metastasis, stromal invasion, and histological grading (P <0.05). 2. COX-2 expression in chronic cervicitis, cervical intraepithelial neoplasia samples varying and cervical cancer rates were 20.0%, 17.6%, 72.3%, cervical cancer and cervical intraepithelial neoplasia like change and chronic cervicitis group, the difference There were significant (P <0.01). The positive rate of CIN group of CIN Ⅰ grade, CIN Ⅱ level and CIN Ⅲ level, respectively, to 12.5%, 25.0%, 40.0%, and three groups of positive expression rate after Fisher exact test, the difference no significant difference (P> 0.05) . The positive expression of COX-2 and cervical cancer patients age, histological type, lymph node metastasis, independent of interstitial infiltration (P> 0.05), and cervical cancer clinical staging, histological grade (P <0.05). 3. MVD values ??respectively in chronic cervicitis, cervical intraepithelial neoplasia-like change, and cervical cancer group 8.72 ± 1.28,24.50 ± 2.43,48.26 ± 3.27: three groups of comparison, the difference was significant (P <0.01). MVD value of the CIN group, CIN Ⅰ, CIN Ⅱ level and CIN Ⅲ grade were 26.27 ± 1.62,27.89 ± 0.87,28.41 ± 1.63; among the three groups the difference was not significant. MVD and cervical cancer patient age, lymph node metastasis and histological grade (P> 0,05), histological type, clinical stage, interstitial infiltration (P <0.05). The positive expression of VEGF and COX-2 in the the cervical cancer MVD median values ??of the upper and lower group comparison, the differences were significant sex (p <0.01). 5 of VEGF and COX-2 expression between cervical consistency (Kappa = 0.375, p = 0.006). Conclusion 1. VEGF in chronic cervicitis - cervical intraepithelial tumor-like change - upward trend, the cervical cancer positive expression of VEGF in cervical cancer high expression with cervical cancer stage, histologic grade and stromal invasion is closely related, suggesting that VEGF may as one of the indicators to judge the degree of malignancy of cervical cancer patients and to predict the prognosis of patients with cervical cancer. 2. COX-2 in chronic cervicitis - cervical intraepithelial neoplasia-like change - cervical cancer positive expression showed an upward trend in grade school, and with clinical stage and tissue, suggesting that COX-2 may be the degree of malignancy as a judge of cervical cancer important indicator of metastasis and prognosis. 3. CD31 is a specific marker of neovascularization, CD31 marker MVD values ??like changes in chronic cervicitis - cervical intraepithelial neoplasia - cervical cancer positive expression of an upward trend the MVD value with cervical cancer clinical staging, histological type and interstitial infiltration, suggesting that the the CD31 marker MVD value can be one of the important indicator as to determine the biological behavior of cervical cancer and clinical progress. 4. VEGF and COX-2 expression in cervical cancer is consistent, and associated with interstitial MVD value, suggesting that they participate in the formation of the occurrence of cervical cancer, the development of interstitial blood vessels.
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