Dissertation > Excellent graduate degree dissertation topics show

Studies on Protective Effect and Its Mechanisms of Se-enriched Lactobacillus on Animals with Subacute Hepatic Injury and Hepatic Fibrosis

Author: YangZuoMan
Tutor: ChenLong
School: Nanjing Normal University
Course: Biochemistry and Molecular Biology
Keywords: Enriched lactobacillus Mice Rats Liver injury Liver fibrosis Intervention Outcome Immunomodulation
CLC: R575
Type: Master's thesis
Year: 2007
Downloads: 180
Quote: 0
Read: Download Dissertation

Abstract


The the chemical contaminants CCl this experiment 4 intraperitoneal injection of ICR mice induced subacute liver injury model and CCl 4 the subcutaneous injection SD rats induced liver fibrosis model, dietary Add enriched lactobacillus protection or vesting animal model, the use of modern biological technology, comprehensive perspective of some of the functional activity of immune cells enriched lactobacillus animals, immune mediators, liver and fat from the of Immunology, cytological, and physiological and toxicological point of view, and hard oxidation state, preliminary enriched lactobacillus CCl 4 -induced liver damage protective effect and CCl 4 -induced liver fibrosis outcome and its mechanism. The results are as follows: 1 enriched lactobacillus intervention subacute liver injury in mice outcome effects and immune regulation mechanism of selection of 30 healthy ICR mice were randomly divided into three groups, namely the control group (C), the CCl 4 < / sub> injection group (CCl 4 group), CCl 4 - selenium-enriched lactobacillus group (CCl4-Se group). Mice subacute liver injury induced by intraperitoneal injection of CCl4, for four consecutive weeks. After the modeling is complete, CCl 4 -Se group was fed a complete feed enriched lactobacillus source preparation vested in five weeks. The results showed that: HE staining, CCl 4 -Se group compared with CCl significantly alleviate 4 group the degree of liver damage. CCl 4 plasma AST, ALT and γ-GT activity than group C, the CCl 4 -Se group compared with CCl 4 groups had reduced ALT activity was significantly lower than CCl 4 group; CCl 4 liver tissue homogenate GSH-Px activity was still lower than in group C at the end of the experiment, and CCl 4 -Se group was significantly higher than the C group and CCl , 4 group; CCl 4 plasma TNF-α and IL-2 levels higher than C Group the CCl 4 -Se group TNF-α levels below the CCl 4 group, IL-2 levels are lower than in the end of the experiment, the C group and CCl 4 < / sub> group. CCl 4 of spleen CD4 and CD8 of T cells (%) and CD4 / from CD8 Group C are rising CD4 (%) was significantly higher than that of the control group, and the CCl 4 -Se group of group C; CCl 4 of spleen T lymphocyte stimulation index was slightly lower than the C group; the CCl 4 -Se group was significantly higher than that the C group and CCl of 4 group. Tip: enriched lactobacillus by improving and improve immune function, effectively alleviate CCl 4 induced subacute liver injury. The 2 enriched lactobacillus on hepatic fibrosis in rats and its immune regulation mechanism study selected 36 healthy SD rats were randomly divided into a control group (C), CCl 4 injection group (CCl 4 group), the CCl 4 - enriched lactobacillus preparations protection group (CCl 4 -Se group). The the CCl 4 group and CCl of 4 -Se group once every three days subcutaneous injection of CCl -induced liver fibrosis, coinjection eight weeks. During, CCl -Se group was fed selenium-enriched lactobacillus full price of feed, the results show: CCl 4 rat liver index highest CCl 4 -Se group declined; CCl 4 plasma AST and ALT activities were higher or significantly higher than that in group C, CCl 4 -Se group between between the two; CCl 4 -Se group rat plasma TP and AL content than CCl 4 group rose; CCl 4 liver tissue homogenate GSH-Px and SOD activity in group C were significantly decreased, while the the CCl 4 -Se group of GSH-Px and SOD activity higher or significantly higher than CCl 4 group; the CCl 4 group MDA content in liver homogenates and Hyp are higher than the C group, the CCl 4 -Se group below CCl 4 group and close to the group C; CCl 4 plasma TNF-α levels compared with the C group, but the the CCl 4 -Se group than in the CCl 4 group. The CCl 4 plasma IL-6 water on average lower than the C group, the CCl 4 -Se group than CCl to 4 group; CCl 4 group T cream the Pakistan cells (%) showed a significant downward trend, while CCl the 4 -Se group compared with the C group a decrease, but significantly higher than CCl 4 group, B-lymphocytes (%) change with T cream cells (%) opposite. CCl 4 group RBC-CR 1 the garlands rate and RBC-IC rosette rate is lower than the C group, and CCl 4 -Se group were high in CCl 4 group, and close to the group C; throughout the experimental period, the CCl 4 group splenic NK cell activity and T lymphocyte stimulation index were lower than in group C, CCl Although 4 -Se group was also lower than in group C, but higher or significantly higher than CCl 4 group; CCl 4 in spleen CD4 and CD8 of T cells (%) and CD4 / CD8 of proportion show large fluctuations enriched lactobacillus can effectively mitigate this change. Tip: enriched lactobacillus by protecting liver tissue antioxidant enzyme activities, improve and enhance immune function and activities to effectively intervene in the occurrence and development of liver fibrosis. 3 enriched lactobacillus intervention liver fibrosis in rats outcome effect immune regulation mechanism study of 38 healthy SD rats were randomly divided into a control group (C), the CCl 4 injection natural outcome group (CCl 4 ) and CCl 4 injection enriched lactobacillus intervention outcome group (CCl 4 -Se group). The CCl 4 group and CCl -Se group injected with CCl 4 2 times a week, 8w establish a model of hepatic fibrosis. Then, the CCl 4 -Se group was fed selenium-enriched lactobacillus full price of feed, 6w observe the outcome of natural and enriched lactobacillus intervention outcome effects. The Show: natural outcome of CCl 4 group and enriched lactobacillus the outcome of CCl preparations intervention 4 -Se group liver index declined, but the CCl -Se group declined significantly stronger than CCl 4 group. Plasma AST and ALT activity declined with a vesting time, CCl are better than 4 -Se group decreased the amplitude and speed the CCl 4 group. CCl 4 liver tissue homogenates although the GSH-Px activity rebounded but still lower or significantly lower than in group C, the CCl 4 -Se group was significantly lower than in the first 2w group C, close to 6w group C level. CCl 4 group the liver homogenates SOD activity during the entire outcome is always the lowest, and CCl 4 -Se group showed a higher level. CCl 4 liver tissue MDA content is always higher than the C group, the CCl 4 -Se group compared CCl 4 group declined. CCl 4 plasma TNF-α levels has decreased, but is always higher than the C group, and the the CCl 4 -Se group, the range between the other two groups. CCl 4 group plasma IL-6 always maintain a high level, and the CCl 4 -Se group and C group close. CCl 4 group and CCl 4 -Se group spleen NK cell activity showed a lower level in the vesting start 2w, and rise with the process of the experiment and the CCl 4 -Se group is always higher than CCl 4 group. The CCl 4 group spleen T lymphocyte stimulation index throughout the vesting period were lower than in group C, and CCl 2w 4 -Se group higher than the C group, to 6w somewhere in between. Throughout the vesting period, CCl 4 the the CD4 rats and CD8 T cells (%) and CD4 of / CD8 proportion of volatile enriched lactobacillus able to effectively adjust to these changes. Hint: the natural outcome of liver fibrosis in rats compared to enriched lactobacillus enhance immune function more effectively accelerated the vesting of liver fibrosis.

Related Dissertations

  1. Research of the Correlation About the Expression of Mfap4 in Liver Fibrosis Tissues and Concentrations of Mfap4 in Peripheral Blood Associated with Liver Pathological Grade,R575.2
  2. Study of Sepia Ink-Astragalus Preparation for Relieving Damage Caused by Chemotherapy,R285.5
  3. The Process Approach and English Writing Teaching in Vocational High Schools,G633.41
  4. Research on Baiyun District Junior High School Students Learning Burnout External Influencing Factors and Its Counseling Strategies,B844.2
  5. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Immature Mouse,S865.13
  6. Research on the Theory and Practice of the Postsecondary Education: Early Intervention Programs in the US,G649.712
  7. Study on Gene Expression of β-defensin1 in Mammary Gland of Mice,S852.4
  8. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  9. Some Pharmacology and Toxicity of Three Prescriptions from Chinese Veterinary Pharmacopoeia,S859.5
  10. Effects of CrPyr on Protein Metabolism in Rats,S865.12
  11. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  12. Apolipoprotein E Gene Knock-out and High-fat Diet on IP3、 IP3R-1 ＆ Aβ Expression in Neurons of Mice’s Hippocampal CA1 ＆ Ca3,R329
  13. Effects of TACI-Ig on MRL/lpr Mice and Activation of JAK1-STAT1 Signaling Pathway in Lupus Nephritis,R363
  14. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  15. The Influence of Early Intervention of Vitamina Combined with Mycobacterium Bovis Bacillus Calmette-Guerin on Lung CD11C+ and Anti-OVA Special IgE Expression in Adult SD Mice Asthma Model,R562.25
  16. Effects and Some Mechanisms of TACI-Ig on Immune Function in MRL/lpr Mice,R593.241
  17. The Effects of Atorvastatin on Expression of Osteopontin in Kidney of the Diabetic Rats Its Molecular Mechanism,R587.2
  18. Maternal Exposure to Fenvalerate in Embryo Accelerated Age-related Changes of Behaviors in CD-1 Mice,R714.2
  19. GC-MS Analysis of Endogenous Metabolites in Plasma of Police Dog after Aflatoxicosis,S858.292
  20. Meridians of Asthma Thread Burying Model Rats IL-4 Such Impact Experimental Study,R245
  21. The Study of the Effects of Isoflavones of Puerarin and Daidzein on Bone Metabolism in Ovariectomized Female Rats,R580

CLC: > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Liver and gall bladder disease
© 2012 www.DissertationTopic.Net  Mobile