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In order established at the cellular level of reactive oxygen species in paraquat (paraquat, PQ) toxicity, the study selection of human acute promyelocytic leukemia HL-60 cell line, HL-60 cells PQ injury model. Changes in the content of malondialdehyde (malondialedhyde, MDA) and total antioxidant capacity (total antioxidation capability, T-AOC) through the inhibition of cell proliferation in the Determination of PQ role by HL-60 cells;; cells superoxide anion (O 2 · - ) and hydrogen peroxide (H 2 O 2 ) content analysis of the PQ in HL-60 the toxic effects of the cell, and preliminary study of glutathione (glutathione, GSH), N-acetyl-L-cysteine ??(N-Acetyl-L-cysteine, NAC), superoxide dismutase (superoxide dismutase, SOD), catalase (catalase, CAT) of PQ protected induction of HL-60 cells poisoning. Main research content and results are as follows: 1. Paraquat induced generation of reactive oxygen species induced HL-60 cell poisoning the direct effect of different concentrations of PQ HL-60 cells, PQ significantly inhibited the proliferation of HL-60 cells, the role of the longer, the more obvious inhibition in a dose dependency; lethal rate the PQ pairs of HL-60 cells also increases with the increasing concentration of the different degrees of lower concentration of the PQ inhibit the growth of HL-60 cells, the high concentration of the PQ directly cause the death of HL-60 cells; HL-60 cell line MDA content increased with the PQ to join, and the determination of the cell total antioxidant capacity of the cells decreased, indicating that the PQ cause oxidative damage to the HL-60 cells, a dose-dependent T-AOC; detection results showed that two types of reactive oxygen species after 12h, PQ treatment of HL-60 cells, the cells O 2 O · - and H 2 2 content with compared to the control group were significantly improved the H 2 O 2 content of the control group compared to than O 2 · - and a dose-dependent manner, This shows that in HL-60 cells PQ poisoning the mid H 2 O 2 plays a critical role in cell damage . 2. Four antioxidants on paraquat induced HL-60 cells poisoning protection observed GSH, NAC, SOD, CAT, PQ-induced protection of the poisoning of the HL-60 cells, they enhance the rate of cell proliferation compared with SOD, GSH and NAC long duration and CAT, and can effectively reduce cell MDA content and enhance the total antioxidant capacity. For PQ poisoning protection role in HL-60 cells, GSH is most obvious, while SOD is the most obvious effect of NAC and CAT in between.
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