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This experiment, the red clover plant regeneration through neutron radiation , resistance to powdery mildew of red clover monoclonal screening and clones as well as the Minshan red clover powdery mildew resistance in physiological three aspects of the study , the following conclusions : red clover seed by different doses of neutron radiation , induction of cotyledon and hypocotyl were lower than the control . 2,4- D 2.0mg · L -1 < / sup> and 6- BA 0.5mg · L -1 < / sup > hormone combinations under cotyledon and hypocotyl highest induction rate . MS 0.06mg / L NAA 0.1mg / L KT sucrose, 2.5% agar, 0.7% for the best differentiation medium , callus pale yellow or light green , loose granular anhydrous Ze of differentiation small amount of stems and hairy root . 1/2 MS 0.05 mg / L NAA sucrose, 1.5% agar, 0.8% for best rooting medium . 2 , neutron radiation the cuttings survived number and survival rate of decline . As the radiation dose increases , the cuttings survived number and the survival rate increased and then decreased , cutting survival rate from 0.66% to 2.27% . Red clover summer cutting survival rate is higher than the autumn . The neutron radiation M1 plant height . As the radiation dose increases the following year cutting seedling number of branches gradually reduce 1.2995Gy processing branch number is higher than that of the control , the number of branches of the remaining processing are lower than that of the control . Cutting seedlings of a creeping red clover . 3 , Minshan the squaring period of red clover inoculated with powdery mildew , flowering stage select resistant plants cuttings through different study M1 plant disease resistance and enzyme activity , chlorophyll, soluble sugar, proline, isoflavones relationship . The results show that the the immune plants SOD activity , POD activity , PAL activity and soluble sugar content , proline content, chlorophyll a and b contents were the highest 223.73U/g.min, 382.95U/g.min, 105.7 U / g.min, 0.0318%, 29.93μg / g, 1.563 mg / g, 0.522 mg / g, is highly susceptible plants 2.75,1.13,1.32,2.41,2.06,1.23,1.50 times . With the weakened resistance , these indicators were dropped . No significant difference in disease resistance among the plant carotenoid content .
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