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Group B rotavirus (group B rotavirus, GBRV) by China Hongtao was first discovered in 1983 , mainly infected adults the pandemic caused large-scale outbreaks of diarrhea many times in our country . The survey results show that of the the crowd group B rotavirus antibody levels , and other countries and regions in the crowd are different titers of group B rotavirus antibodies detected . Exception of China , in the neighboring countries of India , Bangladesh and other countries have also detected from sporadic cases of cholera-like diarrhea group B rotavirus . The purpose of this study was to establish a convenient , fast , sensitive , and specificity of detection technology and methods for the detection of group B rotavirus , help - depth study of the biological characteristics of the group B rotavirus epidemics to learn the laws of detection technology as well as prevention and treatment measures . Isotopically labeled PCR method group B rotavirus nsp5 gene , the sensitivity and specificity of the test probe , and for detecting diarrhea sample . The results showed that the probe sensitivity up to 500pg, specificity , and not unrelated gene hybridization signal . Detected from the Wuhan City, 94 cases of diarrhea specimens case were positive by RT-PCR and sequencing of the test results verified . Group B rotavirus the ADRV of vp7 gene sequence designed synthetic primers , downstream primer 5 ' end-labeled biotin , prepared by PCR method . GBRV vp7 gene fragments biotin-labeled probe , the sensitivity of 50ng, using the same method Preparation of digoxigenin-labeled probe , the sensitivity of 10ng, specificity . Group B rotavirus nucleic acid hybridization method used in this study can be used as a primary screening , can also be used to validate the results of other methods of detection , or for group B rotavirus epidemiological studies .
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