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The Effect and Mechanism of Botulinum Toxin Type A on Electrical Field Stimulation- and Substance Pinduced Contractility in Pyloric and Antral Smooth Muscle in the Rat in Vitro

Author: ZhouZuoZuo
Tutor: HouYiPing
School: Lanzhou University
Course: Human Anatomy and Embryology
Keywords: Botulinum toxin type A Substance P Electrical field stimulation Muscle strips
CLC: R33
Type: Master's thesis
Year: 2007
Downloads: 41
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Abstract

Objective: To observe the botulinum toxin type A (botulinum toxin A, BTX-A) in rat pyloric antrum in vitro the muscle strips spontaneous contraction and electrical field stimulation (electrical field. Stimulation, EFS), substance P (物质 P, SP) caused by pyloric antrum smooth muscle contraction in vitro, and to explore its mechanism. Method: healthy Sprague-Dawley rats, weighing 200 ~~ 250g, male or female. Experiments before fasting for 24 h, drinking water is not limited. Experiment blow to the head After stunning pyloric antral smooth muscle of each one, placed in a constant temperature of 37 ℃ Krebs liquid smooth muscle tank sustained muscle tank supply 95% O ₂ and 5% CO < sub> 2 of the gas mixture, one end of the muscle strips is fixed in the the muscle groove bottom glass hook on the other end is fixed on the tension sensor, the muscle strips were suspended between two silver electrodes, the long axis of the muscle strips connected to the major axis parallel with the electrode, the electrode and the electrical stimulation. Muscle strips incubated in 1g front load, were randomly divided into a control group (control, n = 12), the electrical field stimulation group (electrical field. Stimulation, EFS, n = 12) the EFS of BTX-A group (n = 12), BTX-A group (n = 12), SP group (n = 12), SP of BTX-A group (n = 12), SP NK ₁ receptor antagonist ([d-arg ^{1 , d-phe ^{5 , d-trp ^{7.9 , leu ^{11 ]-substance P) group (n = 12 ). Were added under the conditions of the spontaneous contraction of BTX-A (10 U / ml in), SP (1μM / L), NK ₁ receptor antagonist (1μM / L) and the EFS (frequency 16Hz voltage 35V, pulse width 0.5ms duration of the 60-s), Biolap 420E biological and functional experimental system records the stomach muscle contraction. Results: 1. EFS enhanced (P <0.01) and amplitude enhancement (P <0.05), caused by pyloric smooth muscle and the EFS trigger antral smooth muscle tension and amplitude enhancement (P <0.01); BTX-A inhibition of EFS-induced pyloric antrum smooth muscle tone and amplitude (P <0.01); BTX-A causes pyloric antral smooth muscle tension and reduce the amplitude (P <0.01); SP triggered pyloric smooth muscle amplitude enhanced (P <0.05), the tension no significant difference (P> 0.05), SP lead to the antral muscle tension enhanced (P <0.01) and amplitude enhanced (P <0.01); BTX-A inhibits of SP triggered the pyloric smooth amplitude (P <0.01), does not inhibit SP-induced pyloric smooth muscle tension (P> 0.05), BTX-A inhibit SP-induced the antral muscle tension and amplitude (P <0.01); . Of NK ₁ receptor antagonists inhibit SP triggered the pyloric muscle tension (P <0.01), it does not inhibit SP initiator pyloric smooth amplitude (P> 0.05), NK ₁ by antagonists inhibit SP-induced the antral muscle tension (P <0.01) and amplitude (P <0.01). Conclusion: 1. EFS enhancements the pyloric antral smooth muscle spontaneous contraction; 2. BTX-A inhibits the pyloric antral smooth muscle spontaneous contraction; 3. The BTX-A inhibition of EFS-induced pyloric antrum smooth muscle contraction; suggesting that BTX-A may stimulate muscle contraction inhibition or direct electric field through the inhibition of endogenous neurotransmitter release, thereby inhibiting gastric smooth muscle contraction. 4. SP enhanced the pyloric antral smooth muscle contraction; 5. BTX-A inhibits the SP induced pyloric, antral smooth muscle contraction; 6. NK ₁ receptor antagonist inhibits SP-induced pyloric antrum smooth muscle contraction, prompted SP may play a role, by binding to receptors on the smooth muscle to enhance muscle contraction, and NK receptor antagonists may be combined by antagonistic SP receptor weakened muscle contraction. BTX-A may be through inhibition of synaptic vesicles with the presynaptic membrane fusion, exocytosis, and thus play a role in inhibiting neurotransmitter release may also SP postsynaptic membrane receptor binding has a certain effect, lead to SP triggered the pyloric antrum vitro inhibition of smooth muscle contraction.}}}}

The Effect and Mechanism of Botulinum Toxin Type A on Electrical Field Stimulation- and Substance Pinduced Contractility in Pyloric and Antral Smooth Muscle in the Rat in Vitro

Abstract

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