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Loach (Misgurnus angillicaudatus Cantor) Department Cobitidae animals , meat , or plenary medicine , received are set out in the \Loach 's high nutritional value and curative for many diseases , but less active ingredient ( or parts ) as well as processing methods and pharmacological mechanism of modern methods of loach loach medicinal . This article is extracted from the loach mucus , epidermis , systemic water-soluble Boring loach extraction of polysaccharides , purification and purity identification of the resulting component and system of law with H2O2/Fe2 System Act and the AP-TEMED horse teeth seek polysaccharide semi - pure and refined polysaccharide superoxide anion radicals and antioxidant activity light radicals . Conventional polysaccharide extraction method , i.e. the lye soak extraction and ethanol precipitation, and precipitated anhydrous ethanol , repeatedly washed with acetone , and dried in vacuo loach polysaccharide crude product . Determined by enzyme severing protein chain , combined with classical chemical method (Sevag method ) , deproteinized loach crude polysaccharide . Deproteinized polysaccharide after dialysis was the polysaccharide pure product ( MAP ) . Separated from the epidermis, the body of the loach mucus, extracted into neutral polysaccharides , using a gel filtration column in the high - performance liquid chromatograph check the purity of the polysaccharide extracted , that the polysaccharide extracted as a single component . MAP by UV scan, no nucleic acid (260nm) and protein ( 280nm) characteristic absorption peaks . The IR scans showed typical polysaccharide absorption peaks . Study the loach secretion , the epidermis , systemic polysaccharide O2-· and DPPH · scavenging effect . Utilize H2O2/Fe2 system generates the DPPH · AP - of TEMED system generates O2 -· , respectively, compared Loach secretion clearance rate of the epidermis , the body of polysaccharides on · OH and O2-·; The results show that , MAP has anti - O2- · and DPPH · double effect , and dose-dependent manner . Under the experimental conditions , the the loach body, secretion and epidermal polysaccharide scavenging DPPH · radicals have a strong role in the polysaccharide concentration 0.6ug/ml secreted , the epidermis , systemic polysaccharides on the DPPH · eliminate rate was 65.1 % , 81.2% , 91.2 %; loach systemic secretion and epidermal polysaccharide superoxide radical O2 - , have a strong scavenging effect in the polysaccharide concentration as 1.2ug/ml secreted , the epidermis , systemic polysaccharide of O2- · elimination rate were 72.1% , 85.7% , 86.3 % , prompted the loach polysaccharide has good oxidation resistance .
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