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Objective: based on the bi- dimensional polyacrylamide gel electrophoresis to observe the transplanted tumor H 22 < / sub > animal serum protein , and to explore the tree GAPS GF injection ( GASPGF ) inhibition the serum proteomics mechanism of tumor . Build proteomics technology platform to study the role of anti-tumor medicine . Method : Building animals transplanted tumor H 22 < / sub > model , mice were randomly divided into three groups , the normal group , the tumor group , GAPSGF group . Each serum were separated by SDS-PAGE and 2 - DE technology using Lab work3.0 and PD Quest 6.1 software were analyzed for each group Spectrum observed change . Results : the ① the GAPSGF group and tumor group tumor weight were significantly different ( P < 0.01 ) , tumor inhibition rate was 48.92 % ; ( 2 ) SDS - PAGE results : normal set of sample components 20 , the tumor group 24 , 22 the GAPSGF group ; ( 3 ) 2 - DE results : normal group expressed protein spots 51 , ??55 tumor group , group of 60 GAPSGF . Oncology Group -specific expression of protein spots 31 , co-expression with the normal group but the expression level of a difference of more than 3 times 3 spots ; 30 the GAPSGF group and the normal group co-expressed protein spots . Legible differentially expressed protein spots in the SWISS-PROT database queries , a preliminary judgment of the differentially expressed protein components . Conclusion: ① transplanted tumor ( the H 22 < / sub > ) animal serum proteome changes significantly , including the induction of HSP , the GST of protein expression , raised CAL protein and inhibit the ARG the p21 NMEL expression , these changes the development is closely related to with transplanted tumor occurrence , . Animals the inhibitory effect of the of ② GAPSGF GF injection of transplanted tumors (H 22 ) , the inhibition rate was 48.92% . The ③ GAPSGF GF through impact transplantable tumors in animals serum proteome of the anti-tumor effect ; GAPSGF GF anti-tumor mechanism of molecular biology may be induced ARG , p21 , NMEL , expression , inhibition of HSP expression , down CAL proteins. ( 4 ) In this study, a research platform for research on Chinese anti-tumor effect and technical means
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