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Objective: To explore from the perspective of molecular biology the of Yao Baoke cancer Ling Capsule Antitumor Mechanism . Methods : Experimental Kunming species mice were inoculated with S 180 < / sub > tumor strains Ling capsules after the oral gavage Yao Baoke cancer 11 days after removal of the mouse eye blood , centrifuged to obtain serum were sacrificed and small mice , the removal of the tumor , fixed in formalin . Double- antibody sandwich ELISA assay S 180 tumor-bearing mice peripheral blood TNF -α , IL -2 , IFN -γ content . Using immunohistochemical assay of apoptosis-related gene mutant p53 and Bcl -2 gene expression . Results: ( 1) the impact of tumor-bearing mice serum TNF-α levels : the of Yao Baoke cancer Ling Capsule dose group compared with the model group , serum TNF-α levels have different degrees of increase , which , middle dose group and low dose group , with the model group were significantly different (P <0.05), a statistically significant , while high doses with no significant difference between the model group . (2) the impact of tumor-bearing mice serum IFN-γ were : of Yao Baoke cancer Ling Capsule , the low- dose group compared with the model group , elevated serum IFN-γ were in varying degrees , in the low-dose group with model group , the difference was statistically significant ( P <0.05 ) . (3) the impact of tumor-bearing mice serum IL-2 levels : of Yao Baoke cancer Ling Capsule , in the low-dose group with model group , mice serum IL - 2 level in varying degrees of rise . With the model group , high , middle dose with model group , there were significant differences ( P <0.01 ) , low - dose comparison significant difference ( P <0.05 ) were statistically significant . (4) the impact of the Bcl -2 protein expression of p53 in mice : Yao Bao grams cancer Ling capsules three dose groups of mutant p53 , Bcl - 2 protein expression were weaker than the model group . Conclusion: of Yao Baoke cancer Ling Capsule can significantly increase the peripheral blood of mice TNF-α, IL-2, IFN-γ content , and can inhibit the expression of the mutant gene p53 and Bcl-2 gene , so the role of its anti-tumor the mechanism may be enhanced to improve the immune function of tumor-bearing mice , inhibition of the mutant gene p53 and Bcl-2 gene expression .
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