Dissertation > Excellent graduate degree dissertation topics show

Sensitization Mechanism of Baicalin

Author: TianFengQi
Tutor: QiaoHaiLing
School: Zhengzhou University
Course: Pharmacology
Keywords: Baicalin Allergic reactions IgG antibodies IgE antibodies Guinea pigs
CLC: R285
Type: Master's thesis
Year: 2007
Downloads: 312
Quote: 7
Read: Download Dissertation

Abstract

Baicalin (baicalin) is a kind of flavonoids extracted from the the Labiatae skullcap dry root, skullcap, one of the main active ingredient, a skullcap and a variety of traditional Chinese medicine preparation, such as quality control Shuanghuanglian injection index components . SHL powder injection from the State Administration of Traditional Chinese Medicine in 1992 as the national Chinese medicine hospital emergency department must first batch of proprietary Chinese medicines since has become one of the drugs of choice for TCM treatment of infectious diseases, but its wide range of clinical applications at the same time, adverse reaction reports is also increasing, the report said Shuanghuanglian injection allergic reaction components yellow baicalin. Prepared in vitro to elucidate baicalin induced allergic mechanism the baicalin holoantigen, the establishment of baicalin guinea pig anaphylaxis model. By passive cutaneous anaphylaxis test (PCA) analysis of the regularity of the guinea pig-specific antibodies, enzyme-linked immunosorbent assay (ELISA) and radiation allergen assay (RAST) to determine the 19 sensitized guinea pigs, 16 cases of SHL allergy baicalin specific IgG antibodies in the serum was determined using the RAST the 16 cases Shuanghuanglian allergy serum baicalin specific IgE antibodies, to analyze the role of of baicalin specific IgE and IgG antibodies in allergic reactions. Methods and Results 1. Baicalin antigen preparation of baicalin antigen UV scanning spectra analysis baicalin and bovine serum protein (BSA), human serum albumin (HSA) and poly-L-lysine (PLL) coupled successfully synthesized by the active ester method coupling efficiency 9:1,5:1 and 3:1. 2. Guinea pig anaphylactic shock model baicalin-BSA sensitized guinea pigs after challenge, the animals quickly dyspnea, tumbling to lie down, stop breathing incontinence until death and other symptoms of shock, shock and mortality rates are 90%. The HE staining Show: baicalin sensitized group pulmonary vascular dilatation, congestion, alveolar wall thickening, and normal control group, normal lung tissue, thin walls, no vasculitis pathological changes; the sensitization group of liver tissue edema more obvious and accompanied by inflammatory cells and vascular stasis, displays the typical characteristics of immediate hypersensitivity. 3. Of sensitized guinea pig serum specific IgE and IgG antibodies using the PCA determination of six sensitized guinea pig serum antibody regularity, guinea pigs first 19 days after sensitization began to detect antibodies peaked in the first 31 days, then decreased gradually. Positive PCA reaction after excitation with heating inactivated serum sensitization 3h excited PCA reaction negative after 72 hours, and injected into the body at the same time non-inactivated serum 72h after excitation the PCA reaction positive sensitized guinea pigs while the presence of IgE and IgG type specific antibodies. Spearman correlation analysis, no correlation between IgE and IgG antibody. By ELISA 19 sensitized guinea pigs, and 60 normal healthy guinea pig serum of baicalin specific IgG antibody positive rate was 84%, correlation analysis, the measured specific IgG sensitized guinea pigs baicalin specific IgG levels with PCA The correlation between antibody titer (P <0.05). 4. Mast cell degranulation and experimental the sensitized serum dope processing group isolated ileum contraction after antigen challenge, there are a large number of mast cell membrane is incomplete particles prolapse extracellular degranulation was (72.63 ± 11.45)%, negative control group off particles rate (26.75 ± 6.88)%, see Table 9. Paired samples t-test, model group mast cell degranulation rate significantly higher than that of the negative control group (P <0.01), with the positive control serum dope treated positive rate (68.75 ± 10.99) was not statistically significant (P> 0.05) . The baicalin-BSA sensitized guinea pigs isolated ileum after the baicalin-HSA stimulate contraction obvious that The histamine release about 8μg, negative control group after the baicalin-HSA stimulate contraction of isolated ileum obvious. 5. SHL who are allergic to the serum-specific IgE and IgG antibodies by ELISA and RAST detected in 16 cases of SHL allergy and 60 cases of normal human serum, respectively the baicalin-specific IgE and IgG antibody positive rate of 25% and 37.5%, Independent-Sample t test, those who are allergic specific IgE and IgG antibody levels were higher than normal control group (P <0.01). Spearman correlation analysis between two specific antibodies in the serum of those who are allergic no significant correlation (P> 0.05), the duration of its antibody levels and allergic reactions also no significant correlation (P> 0.05). Conclusion 1. Using active ester Law successfully synthesized the baicalin antigen. 2. Baicalin guinea pig anaphylaxis model. 3. Baicalin guinea pig anaphylactic shock due to mast cell degranulation and the isolated ileum contraction are related to the release of allergy mediators. 4. The baicalin due to the guinea pig and human allergic reactions to their specific IgG and IgE antibodies.

Sensitization Mechanism of Baicalin

Abstract

Related Dissertations