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Vine thirty-seven (Boussingaultia gracilis Miers var.baselloides Bail., Part of the the Basella Division (Basellaceae) the the A. cordifolia genus (Boussingaultia HBK) is a common Chinese herbal medicine, is also one of the new wild vegetable resources in recent years, research and development paper for rattan thirty-seven flowering firm, only to cutting propagation production by more serious geographical restrictions, to establish a preliminary study of its regeneration system;, collected in the field the soil matrix and rattan thirty-seven of nutrition organ samples As Ca, Cd, Fe, Mn, Na, Mg, Hg, Se, Cr, Cu, K, Ni, Zn and Pb, etc. analysis of 15 kinds of metal elements having a strong adsorptive capacity two elements Cd and Hg anti stress tolerance research, in order to provide some theoretical reference for the the vine thirty-seven mineral nutrition research and promotion of industrial vine thirty-seven regeneration system to establish the various stages in the process of adding different combinations and concentrations of plant growth regulating agents research results show that with the stems for the higher rate Iduced; appropriate concentration of MS basal medium 1/2MS; choice of 0.2% PVP as an anti-browning agent can basically prevent browning occurs and does not affect the rate of callus induction and induced effect. the vine thirty-seven callus induction stage, different plant growth regulator composition induced rattan thirty-seven stems with buds callus differentiation play a certain role; cytokinin 6-BA range 1.5 induction effect at ~ 3.0, the optimum concentration for 6-BA2.0; auxin ,2,4-D induction effect is better than NAA, the optimum concentration of 2,4-D1 .0; plant growth regulator add some concentration when the the agent combinations concentration 1/2MS 6-BA2.0 2,4-D 1.0, the highest induction rate, callus growing well. induction of multiple shoots differentiation, the combination of plant growth regulators, to improve induction the best rate, to increase the number of produce buds with good effect; 6-BA concentration 6-BA 1.5 NAA for NAA0.5. induced multiple shoots differentiation, induction rate and the average number of buds maximum and bud growth more evenly, the crops are growing well. induced multiple shoots rooting culture easier, just add a lower concentration of NAA or 2,4-D will be able to promote root differentiation, the concentration of 2,4-D1. 0:00 induction rate reaches a maximum, and the root growth is more uniform. right the vine thirty-seven vegetative organs and growth matrix samples As, Ca, Cd, Fe, Mn, Na, Mg, Hg, Se, Cr, Cu, K, Ni, Zn, and Pb 15 metal elements analysis, the results showed that: 1) vine thirty-seven young shoots, leaves, bulbils and tubers organ 15 kinds of metal elements content and the ability to accumulate in different organs exist significant differences . Leaves K, Ca, Na, Mg, Cd, Mn, Hg, Se, Cu, Zn and Pb content of 11 metal elements and enrichment factor are the four highest organs; young shoots in As, Fe and Cr 3 element content higher than that of the other three organs, the overall showing stems and leaves than the tubers, the middle of the stems and leaves than stems, tubers tubers than the bulbils accumulation characteristics. At the same time, the vegetative organs of the vine thirty-seven strong element enrichment capacity of Cd, Hg, and K, Fe, Cr, and As enrichment capacity is weakest. 2) with reference vegetables compared the vine thirty-seven K, Fe, Mn and Cu content, rich in K and Fe-rich features make the vine thirty-seven mineral nutrition value to improve it as a new type of vegetable development, there is a certain reference value. Meanwhile, a vine thirty-seven different organs, W (K) / W (Na), three groups of W (Zn) / w (Cu), and w (Ca) / w (Mg) ratio are in Clinical Study relatively reasonable range, beneficial to adjust and balance hypertension, coronary heart disease, and other populations in vivo concentration of the corresponding element. Vine thirty-seven as functional vegetable promote. 3) to the national \pollution vegetables and traditional Chinese medicine planting soil environmental requirements. But compliance in the soil environment and no background interference, rattan thirty-seven organ heavy metal contamination of more serious; including on medicinal plants and preparation of import and export of green industry standard \are subject to the As, Hg and Pb polluted in varying degrees, suitable for medicinal purposes only bulbils; press on \significantly grown under natural conditions the vine thirty-seven nutrition organ as a vegetable should be careful. 4) The of rattan thirty-seven pairs Cd stress and combined stress of Cd-Hg role there is a certain degree of patience, but the amount of absorption and accumulation failed to meet the Cd and Hg hyperaccumulator content critical standards; but combined with rattan thirty-seven absolute biomass and adaptation ability, etc., do pioneer plants using the optional governance Cd and Hg contaminated area soil.
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