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Purposes: (1) observed acupuncture phase Sishencong sleep time in rats in a timely manner. (2) to explore acupuncture Sishencong insomnia rat peripheral blood plasma monoamine neurotransmitter norepinephrine (NE) and dopamine (DA), to provide an objective basis for Sishencong treatment of insomnia. Methods: (1) 16 physiological signal recorder measuring pentobarbital sodium-induced sleep time, record the sleep EEG experimental methods to observe the control group, hand pin 30min group, electro-acupuncture 30min group, rats sleep time, and time changes. (2) Wistar rats as subjects, select 50 rats were randomly divided into five groups: control group (blank control group) (10); model control group (model group) (10); stability group (10); hand acupuncture group (10); electro-acupuncture group (10), intraperitoneal injection preparation of chloro-phenylalanine (PCPA) insomnia animal model, orally through acupuncture and stability of the drug group, The rat ocular blood by fluorescence spectrophotometric determination of plasma NE and DA content. Results: Experimental results: (1) the hand acupuncture group and EA group due to sodium pentobarbital the rats sleep time, compared with the control group, there were significant differences (P <0.05). (2) electro-acupuncture group compared with the acupuncture group, sodium pentobarbital-induced sleep time is more obvious, the two groups had significant difference (P <0.05). (3) the hand acupuncture group and the electro-acupuncture group compared with the control group on pentobarbital-induced sleep phase, ie, slow wave sleep the two (SWS2), rapid eye movement sleep (REM) and total sleep Time (TST) was prolonged, there were significant differences (P <0.05). Pairwise comparisons between the three groups, no significant difference (P> 0.05) in one of the slow-wave sleep (SWS1). (4) electro-acupuncture group compared with the hand-acupuncture group, the rats sleep phase SWS2 of, REM and TST time is more obvious, the difference was statistically significant (P <0.05). (5) the hand acupuncture group and the electro-acupuncture group compared with the control group, TST extend more obvious to extend the EA group, there were significant differences (P <0.05). Experiment 2 results: (1) the model group with the control group, plasma NE and DA levels significantly higher than the control group, a significant difference (p <0.05) between the two. (2) the hand acupuncture group and EA group plasma NE and DA content was significantly reduced compared with the model group, a significant difference (p <0.05). (3) electro-acupuncture group compared with the acupuncture group, plasma NE and DA levels reduced more significantly, a significant difference (p <0.05) between the two. (4) stability compared with the model group, plasma NE and DA levels had no significant changes, no significant difference (p> 0.05) between the two. Conclusion: (1) acupuncture can be extended sodium pentobarbital-induced sleep time in rats, can promote sleep, more obvious role of the EA group. (2) Acupuncture adjustable sodium pentobarbital-induced sleep in rats phase, more obvious improvement of the EA group. Can optimize the structure of sleep, improve sleep and improve sleep quality. (3) Acupuncture treatment can reduce insomnia in rat plasma NE and DA content, which may be one of the mechanisms to achieve increased sleep, improve sleep quality. (4) stability of PCPA insomnia rat plasma NE, DA content had no significant effect. This may with clinical patients after taking the insomnia symptoms improved, but the lower the phenomenon of self-satisfaction. (5) from acupuncture PCPA insomnia rat plasma of NE, DA content level point of view, electricity better efficacy for insomnia rats. (6) two experiments, to acupuncture Sishencong improve the efficacy of the rats sleep is for sure. Objective and micro indicators are good echo, the mechanism of acupuncture Sishencong improve sleep architecture in rats may be related to the change of monoamine neurotransmitters in rat plasma content.
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