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The thin layer of electrical chromatography of basic research and its application in pharmaceutical analysis

Author: GeChaoZuo
Tutor: WangDongYuan
School: Shenyang Pharmaceutical University
Course: Drug analysis
Keywords: Thin layer of electrical chromatography RP-18 sintered plate Multiple electroosmosis expand
CLC: R917
Type: Master's thesis
Year: 2008
Downloads: 44
Quote: 0
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Abstract


This paper describes a new type of thin-layer plate - inverting C-18 sintered plate (RP-18 sintered plate ) . Carefully control key heterozygosity of the board can not use the buffer electroosmotic flow for the experiment , which has brought great convenience , saving time , and on this basis can the development of a new expansion method and the use of techniques . This plate has a certain degree of physical strength, to expand the agent may be directly to the brush on the plate to pre-wet , simple , rapid, and save reagents . Expand the process plate dries too large due to Joule heat can be re- directly in the dry parts of wetting , re-energized Expand . This operation can be repeated several times until the spots separated completely , called expand many electroosmosis . The present work together the sintering and key of the TLC plate studies discusses the concentration of reagents and the influence of bonding time on the key heterozygosity . Bonding time extended key heterozygosity increases, within the experimental time rise . Bonding reagent concentration increases, the key heterozygosity increases the experimental concentration also on the rise . The novelty of this work is to get rid of the mobile phase buffered saline , so many electroosmosis commenced by the dream becomes possible ; plate efficiency was affected due to sacrifice some key heterozygosity , therefore , but the nature of the electroosmotic a significant change . The dye mixture nature of the board and use elements of the study to examine the many factors that influence the separation performance . Developing solvent polarity range because of the special nature of this bonding plate , with certain restrictions . Single expand separation decreased , but can be used many times to start a very short period of time and because a single expansion , the total separation has improved the performance and potential of our strategic direction in the field is thus uncertain . This paper discussed . Inverting board of several Chinese medicine Folium and safflower were separated , and the positive phase plate separation results compared . The eluent used by the separation Daqingye ethyl acetate: acetonitrile: water ( 0.5:5:1 ) ; separation safflower eluent, ethyl acetate : acetonitrile : water ( 2.5:5:1 ) .

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CLC: > Medicine, health > Pharmacy > Drug analysis
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