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Expression and Clinical Significance of RUNX3 and TGF-β1 in Epithelial Tumors of Ovary

Author: ChenZhiHui
Tutor: WangXin
School: Central South University
Course: Obstetrics and Gynaecology
Keywords: RUNX3 TGF-β1 Ovarian adenocarcinoma Tumor suppressor gene Pathology
CLC: R737.31
Type: Master's thesis
Year: 2010
Downloads: 26
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Abstract

Objective: To study the RUNX3 protein and TGF-β1 protein in normal ovarian tissue, ovarian cystadenoma, borderline ovarian cystadenoma and ovarian adenocarcinoma expression, to explore the relationship between clinicopathological factors and both ovaries gland cancer role in the process. Material: Hunan Provincial Tumor Hospital and the Maternal and Child Health Hospital, Changsha, 2005-2009, the surgical removal of the 60 cases of epithelial ovarian tumor specimens collected in all patients before surgery were not line chemotherapy, radiotherapy and immunotherapy. Patients age 26-75 (48.12 ± 3.72) years old. Pathologic diagnosis: ovarian cystadenoma (5 cases of serous, mucinous six cases) 11 cases of ovarian cystadenoma (5 cases of serous, mucinous five cases) 10 cases, 39 cases of ovarian cancer In addition selected 15 cases of normal ovarian tissue resection for benign uterine disease. Ovarian cancer FIGO standard surgical - pathologic stage, including stage Ⅰ - Ⅱ 21 cases; 18 cases of Ⅲ - Ⅳ stage. Histological classification according to WHO classification, 13 cases of serous adenocarcinoma, mucinous adenocarcinoma in 12 cases, 14 cases of endometrial adenocarcinoma. Ascites gt; 500 ml of 20 patients, 13 patients with pelvic lymph node metastases. Methods: SP immunohistochemical staining cytoplasm of cells within RUNX3.TGF-β1 are or nuclear membrane, the brown fine particles positive. Each section by two experienced pathologists in five fields under the microscope, counting the proportion of positive cells per high-power field. Positive cells per high-power field rate of 10% to 25% weakly positive (), 26% to 50% in the positive (), gt; 50% strongly positive (). Pathological image applications Motic Image Advanced 3.2 image analysis software, image analysis, and automatic measurement of the optical density of the particles of the positive expression. Each slice randomly selected five horizons, to calculate the mean optical density value. Results: (1) The positive expression rate of Runx3 protein in ovarian adenocarcinoma 21.8% (7/32 patients), the positive rate was significantly lower than the the borderline ovarian cystadenoma 70% (7/10 cases), ovarian Cystadenomatous tumor 81.9% (9/11 cases), normal ovarian tissue, 80% (12/15 cases). Ovarian adenocarcinoma with borderline ovarian cystadenoma, cystadenoma and normal ovarian tissue, there were significant differences (p lt; 0.01). Borderline ovarian cystadenoma, cystadenoma and normal ovarian tissue was no significant difference (P gt; 0.05). Optical density values ??in normal ovarian tissue (830.26 ± 78.34) and cystadenoma in optical density value (819.70 ± 95.08) than in borderline cystadenoma (582.22 ± 44.22) and ovarian adenocarcinoma optical density value ( score into 326.75 ± 41.30, in differentiation of 69.14 ± 17.83, poorly differentiated was 19.08 ± 10.18). And the expression of the optical density with pathological grade (p lt; 0.05). Patients with ovarian adenocarcinoma surgical - pathologic staging, histological type, lymph node metastasis, factors such as age and the amount of ascites no significant sex (P gt; 0.05). (2) TGF-β1 protein in ovarian adenocarcinoma positive expression rate was 17.9% (7/39 cases), the positive rate was significantly lower than the borderline ovarian cystadenoma 80% (8/10 cases), ovarian Cystadenomatous tumor 72.7% (8/11 cases), normal ovarian tissue was 73% (11/15 patients). Ovarian adenocarcinoma with borderline ovarian cystadenoma, cystadenoma and normal ovarian tissue, there were significant differences (p lt; 0.01). Borderline ovarian cystadenoma, cystadenoma and normal ovarian tissue was no significant difference (P gt; 0.05). (3) TGF-β1 protein in normal ovarian tissue optical density (459.28 ± 45.78) and cystadenoma in the optical density values ??(478.16 ± 52.60) than in the borderline Cystadenomatous (387.25 ± 31.37) and ovarian adenocarcinoma. in optical density (high score into 204.22 ± 18.18, the differentiated to 49.98 ± 5.5, poorly differentiated 11.68 ± 1.2). And the expression of the optical density with pathological grade (p lt; 0.05). Patients with ovarian adenocarcinoma surgical - pathologic staging, histological type, lymph node metastasis, factors such as age and the amount of ascites no significant sex (P gt; 0.05). (4) of RUNX3 and TGF-β1 protein expression showed a significant positive correlation (r = 0.786, p = 0.007) of RUNX3 and TGF-β1 protein expression showed the same trend with ovarian histopathological grading changes that organizations Differentiation is lower, the lower their expression. Conclusions: 1 of Runx3 protein and protein expression of TGF-β1 and pathological grading of ovarian adenocarcinoma, suggesting that both related to ovarian cancer. Ovarian adenocarcinoma of Runx3 protein and TGF-β1 protein between correlation exists, they may play a synergistic role in ovarian adenocarcinoma.

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