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Objective: To study of LIMK1 uPAR in colorectal carcinoma and their degree of differentiation, lymph node metastasis, the Dukes staging as well as the correlation between the two. Methods: 87 cases of colorectal cancer specimens (lymph node metastasis in 45 patients), adenoma in 26 cases, cancer surgery off the edge of the normal tissue in 34 cases, a total of 147 cases. Diagnosis and classification of colorectal tumors according to the 2003 WHO histological classification standard. Tissue microarray technology, made of 147 specimens from the tissue microarray. Immunohistochemical detection of colorectal cancer, adenoma and normal tissue LIMK1 and uPAR expression. Results: 5 × 10 chip 3, the sites of morphology can be observed rate of 97.21%. HE staining is uniform, no off-chip, the ectopic and wrinkles; immunohistochemical staining positive positioning clear, clean background. LIMK1 expression in normal tissue, adenomas, colorectal cancer were 20.58%, 38.46%, 75.86% (P lt; 0.01). uPAR expression in normal tissues, adenomas and colorectal cancer expression were 11.53% and 37.93% (P lt; 0.01). Of LIMK1 uPAR expression with age difference was not statistically significant. Colorectal lt; the LIMK1 5.0cm positive rate was 57.14% (20/35), significantly lower than the of ≥ 5.0cm 88.46% (46/52 () P lt; 0.01) Well-differentiated, moderately differentiated, poorly differentiated adenocarcinoma LIMK1 expression rate was 40.00% (4/10) 70.21% (33/47), 96.67% (29/30), there were significant differences (P lt; 0.01). Colorectal cancer A, B of LIMK1 positive rate was 60.98% (25/41) was significantly lower than the C, D of 89.13% (41/46) (P lt; 0.01) Lymph node metastasis positive rate of 97.78% (44/45) without lymph node metastasis was significantly higher than 52.38% (22/42) (P lt; 0.01). lt; 5.0cm large colorectal uPAR-positive rate was 20.00% (7/35), significantly lower than ≥ 5.0cm 50.00% (26/52) (P lt; 0.01). A, B of uPAR-positive rate was 21.95% (9/41) was significantly lower than the C, D of 52.17% (24/46) (P lt; 0.01) Well-differentiated, moderately differentiated, poorly differentiated colorectal cancer uPAR positive expression rate of 20.00% (2/10), 25.53% (12/47) and 63.33% (19/30) (P lt; 0.01) UPAR expression was 55.56% (25/45) lymph node metastasis was significantly higher than that without lymph node metastasis was 19.05% (8/42) (P lt; 0.01) LIMK1 positive group of colorectal cancer uPAR-positive rate was significantly higher than uPAR the LIMK1 negative group positive rate (35.63% / 2.30%), both were positively correlated (r GT; 0, P lt; 0.01) Conclusion: 1.LIMK1, the degree of differentiation of uPAR expression and colorectal cancer, tumor size, clinical stage, lymph node metastasis. Colorectal LIMK1 uPAR and positive correlation between the expression of closely related.
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