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The Research of an Animal Model on Alcohol-induced Osteonecrosis in Short-term
Author: TieWeiBin
Tutor: WangYiSheng
School: Zhengzhou University
Course: Surgery
Keywords: Horse serum Alcohol Osteonecrosis of the femoral head Animal models Short term Rabbits
CLC: R681.8
Type: Master's thesis
Year: 2009
Downloads: 20
Quote: 0
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Abstract
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Background: With the increasing global consumption of alcoholic beverages , alcoholic osteonecrosis of the femoral head in patients is increasing year by year . Alcoholic femoral head necrosis is more common in young and middle-aged male chronic alcoholism , higher morbidity , serious impact on the patient's ability to work and quality of life , has aroused extensive attention of clinicians . With the gradual deepening of alcohol induced femoral head necrosis prevention work to establish an ideal animal model is particularly important . Objective: To investigate ethanol feeding on the rabbit femoral head horse serum sensitization conditions . Methods: 48 male rabbits were randomly divided into four groups , each 12 . Group A: intravenous horse serum 10ml/kg interval of three weeks , the second injection of the same amount of horse serum , two weeks after gavage given saline 10ml / (kg · d). Group B : do not give the horse serum , Group A time synchronization gavage given the potent liquor (volume fraction of 46% ethanol ) 10 ml / ( kg · d ) . Group C: intravenous horse serum 10ml/kg interval of three weeks , the second injection of the same amount of horse serum , two weeks after gavage given potent liquor (volume fraction of 46 % ethanol ) 10 ml / ( kg · d ) . Group D: Group A time synchronization the gavage method only given saline 10 mL / ( kg · d ) . Animals in each group were sacrificed after 4 and 8 weeks after the given alcoholism to observe the results of the femoral head pathological changes : eight weeks after Guanjiu , the C group pathological changes are mainly concentrated in the rabbit femoral head subchondral area , intramedullary reduced hematopoietic tissue , fat substances increased proliferation and hypertrophy of fat cells , trabecular bone thinning , sparse , fracture , trabecular bone area fraction decreases . 4 weeks of empty lacunae increased , compared with the other three groups difference was statistically significant ( P <0.05 ) ; 8 weeks more obvious, and the difference was statistically significant ( P <0.01 ) compared with the other three groups . Conclusion: horse serum sensitization under the conditions given alcohol can cause within a short rabbit alcoholic osteonecrosis of the femoral head .
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CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Bone diseases > Lower limb
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