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Combined Effects of Acetochlor and Copper on Indigenous Microoranism Communities in Phaeozem

Author: ZhangZuoRu
Tutor: ChenXiShi;ZhouQiXing
School: Shenyang Agricultural University
Course: Microbiology
Keywords: Acetochlor Cu2 Combined pollution Black earth Soil bacterial community 165 rDNA sequences Acid-producing Klebsiella spp.
CLC: X172
Type: Master's thesis
Year: 2003
Downloads: 160
Quote: 1
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Abstract


Currently exist in the use of pesticides and the course of the study the following problem: many varieties of pesticides simultaneously or alternately use resulting in combined pollution universal; research on the ecological effects of compound pollution of soil has not been sufficient attention; pesticide combined pollution of soil microbial population structure change its adaptive molecular ecological mechanism combined pollution is still a lack of understanding. Traditional toxicity evaluation tools (microbial biomass, soil physiological indicators) and modern molecular biology techniques (16S rDNA fingerprinting analysis) method of combining single combined pollution of acetochlor and copper metal black micro-ecological stress , including the number of black soil microbial and plant growth of beneficial bacteria, soil dehydrogenase activity and soil respiration intensity; same time, in the establishment of the of soil genomic extracted and 16S rDNA amplification method based on, on the black soil of acetochlor and copper single response with the the combined pollution molecular ecological research; screening resistant strains of acetochlor and its ability to degrade 16S rDNA sequences, but also a strain screening to the degradation of acetochlor Analysis. The main findings are as follows: 1. The the Acetochlor-Cu 2 composite pollution can significantly promote soil respiration enhanced to promote the rate of 14.10%; their inhibition and inhibition of soil dehydrogenase activity compared are the strongest single factor inhibition rate of 93.15% (bacteria), 89.68% (actinomycetes), 24.46% (fungi), 94.97% (authigenic diazotrophs), 89.80% (silicate bacteria), 85.75% (mineralized phosphorus bacteria) 35.03% (soil dehydrogenase activity), showed significant additive effect. 2. The DNA lysate optimization ingredients extract DNA, and nest-PCR amplification and means of variable-temperature annealing of 16S rDNA. 3. Molecules ecological response to environmental pollution, long-term application of pesticides in soil and not applied pesticide clean soil, differences exist, and and the Acetochlor-Cu 2 high-dose combination of contaminated soil bacterial community structure than high similarity, the similarity coefficient of 88%. Three specific bands in the long-term application of pesticides in soil and in the the Acetochlor-Cu 2 high doses of combined pollution appears again and brightness enhancement, while the single pollution and low-dose combined pollution does not appear. From a methodological point of view, the high doses of pollutants on the the soil microbial short-term, acute exposure simulation training pollutants at the molecular level to reflect the long-term impact and the role of environmental and ecological flora contaminants farmland ecological system security The evaluation method reference. The combination of the two technologies can be more accurately and objectively judge the impact of of exogenous pollutants on microbial communities, will also be the future trend of environmental pollution and ecological risk assessment. Screened the biodegradable acetochlor strains 524 from contaminated soil, the degradation rate of up to 62%. Its 165 rDNA sequencing, and found it multiple strains of bacteria produce acid Klebsiella spp (Klebsiellapneumoniae), sequence homology of 99%, preliminary identification of acid-producing Klebsiella spp.

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