Dissertation > Excellent graduate degree dissertation topics show

The Molecular Mechanisms of ZCS for Preventing Sexual Transmission of HIV-1

Author: ZhuLin
Tutor: ChenChaoYin
School: Kunming University of Science and Technology
Course: Biochemical Engineering
Keywords: Sulfated polysaccharide SPR Interaction Drug targets Mechanism of action
CLC: R512.91
Type: Master's thesis
Year: 2009
Downloads: 4
Quote: 0
Read: Download Dissertation

Abstract


ZCS is a poly anionic the esterified polysaccharide sulfate , with an average molecular weight of 24,000 . Live virus in vitro experiments show that , ZCS strongly inhibited HIV-1 replication in vitro . On this basis , in order to clarify the ZCS interaction with HIV-1 molecular mechanism , through the use of the SPR and ELISA techniques preliminary study the interaction of the ZCS with multiple potential targets . The main findings are as follows : (1) ZCS to inhibit gp120 and CD4 binding . SPR system , ZCS can significantly inhibit X4 and R5 two kinds tropism of HIV-1 gp120 and CD4 binding inhibition rates were 82.3 % ( gp120 of III B ) , and 94.3 % (gp 120ADA ) . (2) ZCS with BSA , the BSA fluorescence quenching , quenching type static quenching . ZCS stable binding with BSA complexes with BSA in accordance with the ratio of 1:1.27 . The ZCS role with BSA distance of 4.34nm. ZCS the hydrophobic force main force and BSA . (3) ZCS , with gp120 strong binding . BIAevaluation4.1 software dynamics analysis of gp120 Ⅲ B with ZCS affinity coefficient KD for 136nM ; gp120ADA with ZCS affinity coefficient KD as 442nM . ZCS with gp120 III B affinity than its affinity with gp120ADA . (4) ZCS and strong binding of the V3 variable region . Kinetic analysis by BIAevaluation 4.1 software V3 Ⅲ B with ZCS affinity coefficient KD 33.1nM ; the V3 ADA and ZCS affinity coefficient KD 20.0nM ; V3 GF - 6 ( V3 conserved sequence , GPGRAF) , with ZCS pro and coefficient KD 95.3nM. The ZCS V3 ADA affinity is greater than the V3 Ⅲ B. (5) CD4 ZCS weaker affinity . The BIAevaluation 4.1 Dynamics analysis , the CD4 and ZCS affinity coefficient 1940nM . (6) ZCS is possible to suppress the activity of the HIV-1 reverse transcriptase . The results of calculations by Graphad Prism 5 software ZCS ICso inhibition of HIV- reverse transcriptase activity to 7.437 ± 1.042μg/ml .

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. Study of Interaction between Epoxy Molecules and Carbon Fiber Surface with Molecular Simulation,TB332
  3. The Research on Interaction in the Public Crisis of Government and the News Media in China,D630
  4. Effects of Light, Temperature and Nitrogen on the Growth of Three Kinds of Aquatic Plants,Q945
  5. An Action Research on the Influence of Classroom Interaction on High School Students’ English Speaking Proficiency,G633.41
  6. Research for Social Interaction of Interest-related Groups,C912
  7. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  8. The Research on Mechanism of NaHCO3 in the Prevention and Cure of the Dothiorella Gregaria,S763.7
  9. Research on Structural Vibration of Steam Piping System,TK284.1
  10. Research on Construction Accident Factors’ Interaction Relations,TU714
  11. Yeast Two-Hybrid Screening of 14-3-3-Interacted Proteins During Early Cotton Fiber Development,S562
  12. On Governance Optimization in the Vision of the Network Politic,D630
  13. Research on Teaching Interaction of Collaborative Knowledge Building in Web Environment,G434
  14. A Study of Theory and Practice in Interatcion between Primary Schools and Community Areas,G621
  15. Research of the Partnerships Construction of Schools and Communities,G637
  16. Hydrophilic structure of different types of surface -modified polyurethane material and coagulation 12 factor nine peptide fragments,and fibrinogen P1 fragment of computer simulation of the interaction,O631.3
  17. Identification and Fuctional Study of Genes Induced in Xanthomonas Oryzae PV. Oryzae by Its Host Plant,S435.111.4
  18. Sequence Variability Within ORF94 and Interactions between Evelope Protein of White Spot Syndrome Virus (WSSV),S945.1
  19. Screening of Proteins Interact with Hepatocyte Nuclear Factor3β by IP-MS and Primary Study of Function,R341
  20. Nuclear Dot Protein NDP52 Bind to Tumor Necrosis Factor Receptor Associated Factor 6 and Their Clinical Significance of Research,R363
  21. Effective of the Interaction between NDP52 and TRAF6 and TRAFs on NF-κB Signal Pathway,R363

CLC: > Medicine, health > Internal Medicine > Infectious disease > Viral infections > Acquired Immune Deficiency Syndrome ( AIDS AIDS)
© 2012 www.DissertationTopic.Net  Mobile