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This test is carried out in two phases, to explore different levels of yeast culture material (YC), antibiotic production performance of broilers (1 to 4 weeks), gut microflora of immune function, endocrine and nutrient utilization the YC, Saccharicterpenin, probiotics, mannose oligosaccharides (MOS) control the effects of antibiotics on broiler late (5-7 weeks) of E. coli, integrated assessment of the YC application of significance, and its in-depth development, provide theoretical support . A test: select health, early weight similar to a 210-day-old Avian broiler male chicks were randomly divided into seven groups, each group with three replications, each repeated 10 feather chicken in the basal diet (not add any drugs) YC (0, 0.1%, 0.3%, 0.5%, 0.7%, 0.9%) and antibiotics (5mg/kg flavomycin) is pre-production on broiler performance, gut microflora and immune function and endocrine. Determination of weight of each set of test chickens per week, balance sheet feed consumption and feed conversion ratio. 21-day, three-day metabolic tests. 14 days of age, respectively, 21 days of age in each group extracted chicken neck vein blood serum was separated after the determination of the where T 3 T 4 ; slaughtered and dissected the test chickens free spleen, the bursa and left thymus, weighed. 21 days of age in each group extracted 3 chickens jugular vein blood collection, one of the IgA, IgG, IgM, and physiological and biochemical indicators (total protein, globulin, albumin, calcium, phosphorus) were determined after separation of the serum; take cecal contents of E. coli , Bifidobacterium Determination. 28 days of age take cecal contents of each extract 3 chickens jugular vein blood collection SOD levels measured after separation of the serum total cholesterol; slaughter and anatomical trials chicken, free spleen, the bursa and left thymus, weighed; E. coli, Bifidobacterium the determination; clipping duodenum bowel determine structural changes intestinalis; pectoral frozen after lethal After analyte wherein the total cholesterol content. A test results show that: the dietary supplementation of 5mg/kg flavomycin significantly increased (P <0.05) broiler early daily gain and feed conversion rate, compared with the control group, the average daily gain increased by 9.11%, feed conversion decreased by 10.37%. The addition of 0.3% YC broiler weight gain and feed conversion ratio slightly improved improve broiler weight 1.97%, but no significant difference (P> 0.05) statistical difference between the whole term feed intake groups was not significant (P> 0.05), each the YC group of broilers pre-production performance compared to the control group, the difference was not significant (P> 0.05). Added 0.3% YC can significantly improve broiler utilization of phosphorus in the diet (P <0.05), compared with the control group, and antibiotics, increased by 20.47% and 14.81%, respectively, and no significant impact on other nutrients utilization. Added 0.3% YC can significantly improve broiler serum IgG levels (P <0.05), compared with the control group, 12.32% increase in serum IgA, IgM and immune organs no significant impact. 0.3% YC and antibiotics can significantly improve the the broiler intestinal bifidobacteria quantities (P <0.05) reduced the number of broiler cecum Escherichia coli (1 to 4 weeks), to reduce diarrhea, decreased intestinal ammonia concentration, improve the intestinal mucosa variable length structure microvilli. 0.3% YC add significantly improve broiler serum T 3 level, compared with the control group and the antibiotics were increased by 8.38% and 8.16% (P <0.05), the impact of the of other endocrine observed indicators not significant. Dietary supplementation of YC Agronomy, Northeast Agricultural University, master's degree thesis a raise broiler antioxidation function and to reduce serum the muscle total cholesterol content trend (P> 0.05). Phase II trials: after 28 days of age, each set of adjustments for each group of six birds, the control group, 0.3% and 0.5% group, antibiotic group remains unchanged, the remaining (0.l%, 0 .7%, 0.9% YC) group were changed to add 750mg/kg sugar anvil prime, 0 *% Probiotics and 750mg/kgMOS of test, study different alternatives to antibiotics, infection of E. coli on broiler late inhibition. Test from 29 days to 35 days of age, all chickens droplets port 10 'E. coli inoculation, observed the morbidity and mortality of chickens each extract 3 chickens jugular blood was determined by physiological and biochemical indicators: 40 days, whichever The cecal contents isolation and culture of E. coli intestinal structural changes in the determination of broilers infected with E. coli after clipping duodenum. Phase II trial results showed that: YC, dietary supplementation the prebiotics, MOS, antibiotic significantly reduce the morbidity and mortality (P 0.05). The antibiotics significantly reduces the broilers infected with E. coli after the wall thickness (P <0 knife 5), o. 5% * c group with the antibiotic group, no significant difference (> 0.05).
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