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Jiawei Dangguibuxue Tang Has an Impact on the Expression of Renal Tissue in Rats of Hyperlipidemia CTGF Protein and mRNA
Author: YeZhongWei
Tutor: ZhengXiaoWei
School: Zhejiang University of Traditional Chinese Medicine
Course: Chinese medicine
Keywords: Hyperlipidemia / TCM therapy Kidney disease / TCM therapy DBD / pharmacology
CLC: R285.5
Type: Master's thesis
Year: 2010
Downloads: 48
Quote: 0
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Abstract
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Purpose : flavored Dangguibuxuetang , hyperlipidemia renal tissue connective tissue growth factor (CTGF) protein and mRNA expression of point of view , to explore its mechanism of action of anti - kidney damage . Method : flavored Dangguibuxuetang , high fat diet -induced kidney damage model for intervention , rats were randomly divided into normal group, model group , simvastatin group , the modified Danggui blood soup is low , medium and high dose groups each treatment group for intervention after 12 weeks of administration , immunohistochemistry technology semi-quantitative detection of CTGF protein expression levels of fluorescence quantitative reverse transcription polymerase chain reaction ( RT - PCR ) method detection CTGFmRNA the expression levels from each group other CTGF protein and mRNA expression levels of hyperlipidemia induced the kidney damage and flavored Dangguibuxuetang , its mechanism of action . Results: Immunohistochemistry results showed that the model of renal tissue CTGF protein expression was significantly higher than the normal group ( P lt ; 0.01 ) , each treatment group rat CTGF protein expression in varying degrees to reduce , the soup flavored Dangguibuxue high dose The most obvious group , followed by the positive control group , traditional Chinese medicine dose group and low dose group, fluorescent quantitative reverse transcription polymerase chain reaction ( RT - PCR ) test results show renal tissue of the model group was significantly higher than the normal group in CTGFmRNA have different degrees of reduction ( P lt ; 0.01 ) , the expression of each the treated rats CTGFmRNA the most obvious , of flavored Dangguibuxue soup high dose group , followed by the positive control group , dose group of traditional Chinese medicine , Chinese medicine low dose group . Conclusion: the flavored Dangguibuxuetang prevention and treatment of hyperlipidemia induced nephropathy mechanism may be lowered CTGF protein and mRNA expression .
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