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Objective: Decoction of the seminiferous Polysaccharides Removal animal experiments, polycystic ovary syndrome rats Compare polysaccharide spermatogenic Decoction and its Polysaccharides Removal aqueous solution ovulation induction, and make evaluation, for the guidance of medicine preparation, and to further clarify the active ingredient. Methods: 70 days old, clean SD female rats 60, weight 240g ± 20g, normal control group (n = 10), and the remaining 50 to Poresky method HCG (3IU / d) and insulin in the (dose 0.5IU / d increased 6IU / d and maintained to the end of modeling) subcutaneous injection of 23 days induced the formation of polycystic ovary syndrome, based on body weight after the end of modeling the model rats were divided into model group and treatment group (including positive drug group, the spermatogenic decoction on the group polysaccharide group and spermatogenic soup to the polysaccharide treated) (n = 8), normal rats selected based on body weight 8 to continue the experiment, the remaining rats were removed. Give grouped according to the requirements of different drugs were administered once a day for 14 consecutive days. The last day after the administration, all rats were fasted overnight, the next day decapitated, blood, determination of serum E2, P, T content. Dissected uterus and ovaries and record the weight of formalin-fixed ovarian tissue to prepare sliced ??observation ovarian pathological. Results: polycystic ovary syndrome model rats with normal group serum E2, P significantly reduced P LT serum levels of T; 0.01, significantly increased P LT; 0.05. Germ soup to polysaccharide in rats treated serum E2, P content with the model group rats increased significantly reduced serum levels of T and P LT; 0.01, the difference was significant; the spermatogenic soup go polysaccharide treated serum E2 , P, T content with spermatogenic Decoction group increased P GT; 0.05, no significant difference. Polysaccharide rats compared with the model group serum E2, P content increased, reduced serum levels of T but P gt; 0.05, no significant difference. Model group were the uterine weight lower than normal group, but P gt; 0.05, the difference was not statistically significant. Polysaccharide group rats after treatment with the normal group increased uterine weight P lt; 0.05 difference was significant. The model rats ovarian weight performance abnormal compared with normal group weight significantly increased P LT; 0.05, the difference was significant. Ovarian weight and model group the spermatogenic soup to polysaccharide rats after treatment comparison mitigate P LT; 0.05 significant difference; the spermatogenic soup to the polysaccharide rats spermatogenic Decoction group increase in ovarian weight, P gt; 0.05 The difference was not statistically significant. Polysaccharide group rat ovarian weight compared with model group p gt; 0.05, the difference was not statistically significant conclusions: (1) Poresky method PCOS animal model features serum E2, P decreased serum T was significantly higher the rat ovarian volume and weight increase. (2) the seminiferous soup go to the polysaccharide treatment does not affect the overall drug efficacy, due to the concentration of the active ingredient to increase serum E2, P's enhanced power efficiency, thereby enhancing the regulation of ovarian function, in order to achieve a stronger role in ovulation induction . (3) polysaccharide with increased polycystic ovary syndrome serum E2, P and reduce the role of serum T does not have a role in regulation of ovarian function, but can promote the development of rat uterus.
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