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The purpose of a slow transit constipation rat colon 5-HT analyzed the changes in the characteristics of excitatory receptors 3,4 study provides a theoretical basis for the treatment of constipation drugs. Study Simotang slow transit colon excitatory receptors impact of 5-HT3, 4, explore Qi and Chinese medicine to improve the mechanism of action of colonic motility, lay the foundation for the traditional Chinese medicine drug research. Methods weight 200 ± 20g SD rats 60, were randomly divided into 2 groups: distilled water control group of 12, model group (n = 48), rhubarb gavage modeling the starting dose 1000mg/Kg / day, the final dose reached approximately 9000mg/Kg / day, control group received the same volume of distilled water, to complete the three cycles, and shared 45 days. After stopping a week removed from the model group were randomly after 12 rats, distilled water and 12 control group at the same time can not help but water fasting 24 hours, sacrificed measured intestinal length carbon powder advance and promote the rate, remove the specimen. Another 36 rats were then randomly divided into three groups, a group of model group fed with distilled water control, and the other two groups for the the Chinese intervention group of high-dose group and low dose group. The Chinese intervention group orally Simotang, high dose group containing crude drug 1.14g, the low-dose group for 0.57 g continuous gavage for two weeks and were killed after rate measured carbon propelling remove specimens, formalin-fixed paraffin-embedded after . EnVison were detected groups paraffin sections 5-HT3, 4 receptor protein expression, and finally a computer-assisted image analysis and statistical analysis. 1 model group carbon powder propulsion length and propulsion rate (63.45 ± 3.54cm and 42.78 ± 2.96%), respectively, were less than the control group (respectively 91.25 ± 11.23cm and 66.12 ± 7.64%) was statistically significant (P carbon powder advance length Chinese intervention group high-dose group (74.45 ± 16.28cm) higher than the model group (58.78 ± 8.02 cm) 'statistically significant (P lt; 0.05), carbon powder advance rate (60.49 lt; 0.05); ± 6.43%) was significantly higher than the model group (44.65 ± 9.00%), a significant difference (P lt; 0.01); charcoal powder advance the length of the low-dose group and advancing rate (70.50 ± 14.77 cm and 54.45 ± 8.09%, respectively) were higher than the model group (58.78 ± 8.02 cm and 44.65 ± 9.00%), carbon powder to promote high-dose group were statistically significant (P lt; 0.05); higher than the low-dose group (P lt; 0.05), advancing The length of the difference was not statistically significant. Model set of 5-HT3 excitatory receptor expression levels (61.60 ± 15.00) than the control group (100.82 ± 9.24), a significant difference (P lt; 0.01); 5-HT3 excitability high dose group of Chinese intervention group receptor relative expression level (87.11 ± 19.69) than the model group, there were significant differences (P lt; 0.01), the low-dose group (83.18 ± 17.01) 5-HT4 receptor expression levels in the high dose group (95.71 ± 17.90 ), model group (70.74 ± 16.72) were significantly increased relative to the normal group (112.62 ± 13.56), there is a significant difference (P lt; 0.01). Low-dose group (89.33 ± 7.84) than the model group was statistically significant (P lt; 0.05). Conclusion slow transit constipation rat colonic the excitatory receptor expression levels of 5-HT decreased, suggesting that the 5-HT3, 4 may affect the occurrence of slow transit constipation. The increased excitatory receptors Simotang 5-HT3, 4 expression levels, suggesting that qi medicine may improve colonic motility by upregulating the expression of the excitatory receptor 5-HT3, 4.
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