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Tree tongue polysaccharide GF is a polysaccharide extracted from the tree tongue dry fruiting body composition, and studies have shown that tree tongue polysaccharide GF has anti-tumor, and the role of the immune system. Therefore, the tree tongue polysaccharide GF Extraction, separation and purification and composition of the preliminary research has greater theoretical significance and application value, the energy for applanatum polysaccharide activity and development of new drugs and the applanatum quality control to provide the basis of the technical theory. Objective: systematic study of applanatum polysaccharide GF clarify applanatum polysaccharide GF extraction, separation and purification methods, preliminary study of its composition, to provide a scientific basis for the study of polysaccharide activity and new drugs. Methods: 1. Boiled alcohol precipitation tree tongue dry fruiting bodies were extracted tree tongue polysaccharide GF. Sevage law in addition to protein, 10% H2O2 oxidative bleaching, dialysis. Tree tongue polysaccharide GF by UV detection purified by DEAE-52 cellulose ion exchange column chromatography. 3. Flow divided by the freezing point depression method determination of the relative molecular mass, and then analyzed by thin layer chromatography. 4 using phenol - sulfuric Determination tree tongue polysaccharide GFa, GFb GFc, GFd the content. Results: 1. Applanatum extract, results showed that the extraction rate is 2.126 ‰. . Sevage exception protein, obtained after 10% H2O2 oxidative decoloration purified applanatum polysaccharide GF, and then by DEAE-52 cellulose ion exchange column chromatography, initially to obtain four kinds of flow stars, respectively GFa, GFB, GFC GFd. Measured by freezing point depression method, the GFa, GFb GFc GFd relative molecular mass of 1159.33g/mol, 3654.54 g / mol, 3821.19g/mol 1246.85 g / mol. The various fractions obtained by thin layer chromatography analysis, GFa by D-galactose, L-rhamnose two monosaccharides, GFB from D-glucose, D-arabinose, D-galactose and L-rhamnose sugar four monosaccharide composition, GFC is D-glucose, D-xylose and D-arabinose three kinds of monosaccharide composition the GfD is from D-glucose, L-rhamnose two monosaccharides. Tree tongue polysaccharide GFa, GFb, GFc, GFd determination of its content were 15.42%, 17.25%, 18.52%, 14.72%, the best hydrolysis time 6h, 5h, 5h, 6h, respectively. Conclusion: 1. Applanatum polysaccharide GF extraction rate of 2.126 ‰. Fraction GFa relative molecular mass of GFb GFc, GFd 1159.33g/mol, 3654.54 g / mol, 3821.19g/mol 1246.85 g / mol. 3. GFa by D-galactose, L-rhamnose two monosaccharides GFB is D-glucose, D-arabinose, D-galactose, and L-rhamnose, four kinds of monosaccharides, GFC from D-glucose, D-xylose and D-arabinose three single sugar composition GfD from D-glucose, L-rhamnose two monosaccharides. 4 tree tongue polysaccharide GFa, the GFb GFc, GFd content were 15.42%, 17.25%, 18.52%, 14.72%, the best hydrolysis time 6h, 5h, 5h, 6h, respectively.
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