|
Objective : To observe the the Tongfu soup ALI rat lung , intestinal tissue TGF-β1 . Explore the mechanism of the Tongfu soup ALI 's role , to provide experimental evidence for the Lung and Large Intestine \Methods : 180 rats were randomly divided into six groups , normal control group , model group , dexamethasone group , Chinese low-dose group , the dose group of traditional Chinese medicine , traditional Chinese medicine high- dose group , 30 / group . Tail vein injection of LPS copy ALI model , traditional Chinese medicine each dose group was given half an hour later , the the dexamethasone group of intraperitoneal injection treatment , 1,2,6 hours after treatment , each 10 rats were sacrificed . Light microscope lung, intestinal histological changes ; ELISA method synchronous detection of TGF-β1 in the lung , intestinal tissue . Results: 1 . Macroscopic model group, lung , intestinal tissue dark red color , swelling , bleeding , the treatment group compared with the model group relief . Light microscope to see the model lung , intestinal inflammatory cell infiltration , edema , hyperemia , structural damage , the degree mitigate the treatment group compared with the model group . 3 model group, lung , intestinal tissue content of TGF-β1 within the first six hours with time to grow. 4.ALI lung , intestinal tissue : TGF-β1 content model group is higher than the normal control group was statistically significant ( P lt; 0.05 ) ; dexamethasone group , traditional Chinese medicine , the dose of TGF-β1 levels were lower than the model group was statistically significant (P lt; 0.05); Chinese medicine low dose of TGF-β1 content higher than the dexamethasone group was statistically significant ( P lt; 0.05 ) ; dose group of traditional Chinese medicine , traditional Chinese medicine high dose group of TGF -β1 no difference in content dexamethasone group , no statistical significance ( P gt ; 0.05) ; Chinese medicine in dose group, herbal high dose of TGF-β1 levels were lower than the Chinese medicine low dose group , with statistical significance ( P lt ; 0.05 ) ; Chinese medicine high dose of TGF-β1 content with traditional Chinese medicine in the dose group no difference , no statistical significance ( P gt ; 0.05) . Conclusion: Tongfu soup can reduce the content of TGF-β1 in the lung tissue of ALI rats in the high dose group effect is better . Tongfu soup can reduce ALI rat intestinal tissue content of TGF-β1 to good effect in the high- dose group .
|