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Objective: The topics to the overall results of animal studies, chronic restraint stress method copy prem-enstrual syndrome premenstrual syndrome (PMS) qi depression syndrome rat model, observation model rat serum containing neonatal rat hippocampus the nerve the yuan ERp and related genes 5-HT1AR ,5-HTT, MAO-A / B mRNA levels, from the level of gene transcription to investigate the pathogenesis of the PMS liver qi depression syndrome and treatment of PMS qi depression syndrome mechanism for premenstrual Granules by PMS qi depression syndrome clinical treatment to provide reliable experimental evidence. Methods: vaginal smear microscopy screening non-accepted period of experiments on rats entered in the estrous cycle, were randomly divided into normal control group, the PMS liver qi depression syndrome model group and premenstrual Granules dose group; modeling and drug intervention completed later, the rats were sacrificed collected serum, spare; using cell culture techniques, application of the treatment groups serum on cultured rat hippocampal neurons intervention, and semi-quantitative RT-PCR method, respectively, of the cells in each group ERβ and related Gene 5-HT1AR ,5-Htt, MAO-A / B mRNA levels were detected. Results: chronic restraint stress modeling can effectively simulate the characteristics of women premenstrual depression, the symptoms of depression disappeared after; RT-PCR test results show that, compared with the normal group, model group significantly higher serum rat hippocampal neurons ERβ , MAO-A mRNA levels (p lt; 0.05), and significantly reduce rat neuronal element 5-HT1AR ,5-HTT mRNA level (p lt; 0.05), the former SHU particles containing serum can significantly improve the PMS liver Melancholia model serum intervention hippocampal neurons ERβ, MAO-A and 5-HT1AR ,5-HTT gene transcription level exception (p lt; 0.05); MAO-B mRNA levels of the normal control group, PMS liver depression syndrome model group and premenstrual Shu particles administered serum after the intervention, there was no significant difference (p gt; 0.05). Conclusion: Chronic restraint stress method successfully copied a rat model of the PMS liver qi depression syndrome; abnormal changes of the serum of each treatment group intervention rat hippocampal neurons ERβ and related gene 5-HT1AR ,5-HTT, MAO-A mRNA levels Tip ERβ and related genes 5-HT1AR, 5-HTT, MAO-A liver prescription Granules target of premenstrual SHU particles may affect ERβ and related genes 5-HT1AR, 5-HTT of MAO-tune the transcription of AmRNA play a therapeutic role, little correlation the MAOB premenstrual depression.
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