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Therapeutic drug monitoring (therapeutic drug monitoring, TDM) has been carried out in our country for 20 years, during this period, many scholars use various theories and technologies of modern analytical instruments measured drug concentration in body fluids (blood, urine, etc.). Nonvolatile memories at home and abroad since the 1980s, research on Chinese medicine and Compound pharmacokinetics (Pharmacokinetics, PK), a leading Japanese scholars abroad biological prescription analysis has become more sophisticated, the use of nuclear magnetic resonance, gas liquid chromatography technology and advanced analytical tools, such as a mass spectrometer can do to each other absorbed into the body components in the the trace even trace amounts range of qualitative and quantitative various online equipment such as microdialysis, can reduce subjects suffering under the premise of complete pharmacokinetic sampling or measurement process. And domestic scholars and its almost simultaneous development first proposed in 1991, Professor Huang Xi Treatment Pharmacokinetics (Pharmacokinetics of traditional Chinese Syndrome and recipe RS-PK) ideas, the research group of modern analytical techniques with a liquid, gas, etc. The qualitative and quantitative determination of the chemical composition of the blood after taking Chinese proprietary, and studied their PK. After the proposed \the material basis of in vivo efficacy of biological prescription law and its interaction with the body. research ideas and methods will break through the backwardness of our country in the prescription clinical PK studies, and gradually solve the compound effects, quick, effective in treatment of repetitive problems Currently, foreign reports with blood, urine concentration of biological prescription clinical pharmacokinetic (PK) and its related research more domestic Chinese proprietary and Botanical extractant compound PK reported the detection of blood concentration urine concentration detection prescriptions PK study very little. According to our research group retrieved more than 10 million prescriptions, about 17 blood (urine) drug concentration preliminary clinical pharmacokinetic ( TD than no reported clinical TDM called) and its related research, 9 including Japan, China, 8: dozens literature, only one animal; Japan has been the project in 1995 funded prescription pharmacokinetics, the above condition shows that the backward prescription clinical PK studies in China and the challenges facing the international, TDM, will help us to quickly catch up and occupy the international prescriptions PK study leading the experiment that is the foundation in our experiments laboratory research ideas, on an experimental basis, and the analysis of the situation of the research, intended to establish the use of gas chromatography (GC-FID) borneol JiuXinWan in human urine, qualitative and quantitative Isobornyl its concentration The research methodology; as a tool for drug JiuXinWan borneol Isoborneol provide reliable experimental data measured in Coronary Heart Disease patients and healthy volunteers in urine concentration for TCMR-TDM Methods : In this experiment, HP4890D gas chromatograph (USA) determination capillary column of HP-5 quartz elastic (1 X053mmID) chromatographic conditions: urinary a column temperature 75t, inlet temperature 140 C, the gasification room temperature 150OC, the flow rate was the 8.lml/min; blood column temperature 85C inlet temperature of 230 oC, the gasification room temperature 240 ° C; nitrogen as a carrier gas state.), a flow rate of 8. sml / min, hydrogen ions fire · 5 · scissors back cover the huge yarn Xiang's degree Nongong flame detection device (FID). Selected six cases of coronary heart disease in patients with blood stasis syndrome and six cases of healthy people, its sublingual Jiuxin pill after 10,30,60,90 minutes to collect blood and urine samples; the urine pretreatment selection of n-hexane: ether (l: l) direct extraction method, plasma pretreatment selection of n-hexane: chloroform (1: l) direct extraction, the internal standard method, internal standard substance chosen prime. Results: healthy volunteers after sublingual JiuXinWan in Sichuan, urine concentration of 30, 60, 90 minutes vivo borneol were l. 148f0.240, 2.766fi. 731,5.982 disabilities 4.17s 5.905 soil 4.614 11 iml; plasma concentration were 15.82 disabilities of 9.89,18.68 disabilities 14.55,10.63 soil 4.49,6 .16 disabilities 1.82 ug / ml; coronary heart disease in patients with blood stasis syndrome the sublingual JiuXinWan in urine concentration of 10, 30, 60, 90 minutes when the body borneol 1.056 soil 0.549,4 .939 ± 2.165, 8.174 ± 5. * 7,5.6 * Guests 3.509p ga.: 0.676 the urine concentration Isoborneol were on 0.231,1.039 on 0.124,0.829 soil 0.291,0.945 Guests 0.614p g / ml; the borneol blood concentration of 55.65 door. 89,41.07 soil 32.69,28.17 Tumen. 54,12.83 soil 6.33 pg / ml; the Isoborneol plasma concentration were 50.95 t 14.64,34.57 130. The ZI, 28.64 Guests 6.91,11.99 disabilities 4.46ug/ml. Significant difference (p <0.05) compared with the healthy group, 30, 60 minutes. Conclusion: l In this study, for the first time to explore the the borneol detected in the urine after people taking JiuXinWan methodology. Pretreatment and GC detection method of the experiment is simple, high sensitivity, good reproducibility, for JiuXinWan borneol quantitative detection and pharmacokinetics in human urine metabolic contained?
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