Dissertation > Excellent graduate degree dissertation topics show

A Study on the Function of Dentin Matrix Protein 1 during Tooth Development and Dental Pulp Repair after Injury

Author: ZuoJianLiang
Tutor: WuBuLing
School: Fourth Military Medical University
Course: Clinical Stomatology
Keywords: Dentin matrix protein 1 Tooth development Alkaline phosphatase Reparative dentin
CLC: R780.2
Type: Master's thesis
Year: 2003
Downloads: 79
Quote: 0
Read: Download Dissertation

Abstract

In recent years, the role of dentin non-collagenous proteins (NCPs) in tooth development and pulp damage repair has become the hotspot of the pulp biology research, NCPs dentin specific protein, mineralized tissue-specific protein, protein polysaccharides, glycosaminoglycans, a small amount of phospholipids and some growth factors. They induced odontoblast differentiation and dentin mineralization increasing attention. So far, most research is focused on the dentin-specific protein, mineralized tissue-specific proteins in tooth development less functional studies. How they are involved in tooth development, how to adjust the dentin mineralization process, whether to participate in the the pulp damage repair process, requires further study. Dentin matrix protein 1 (dentin matrix protein 1, DMP1) is one of the important members of the mineralized tissue-specific protein, is a novel serine-rich acidic phosphorylated protein, 1/3 of the door in its composition aspartic acid and glutamic acid, the isoelectric point of 3.68. In different species, the the DMP1 gene the gene structure is similar to having a high degree of homology, and there are some highly conserved regions. DMP1 and bone sialoprotein (BSP) and osteopontin (OPN) is structurally similar DMP1 function and biological activity may be associated with both are similar. Existing research shows that: DMP1 in tooth germ mainly expressed in odontoblasts, overexpression of DMP1 can induce the differentiation of these cells and the formation of odontoblast-like cells in the pluripotent cells derived mesenchymal cells, and can promote the formation of mineralized nodules. Suggesting that the might of its important role in the formation and mineralization of dentin. Fourth Military Medical University, a master's degree thesis on the DMP are mainly concentrated in the biological structure and physicochemical properties, less research for the DMP function reports of DMP in the dentin and pulp in the role and mechanism of injury and repair very less. The experimental monoclonal antibody anti-DMP prepared by Gao Jie, explore is how to form DMP odontoblast regulation by which factors in the formation, as well as in pulpal repair, and the use of in vitro culture abroad newly created small immortalized mouse cloning dentin cell line MDPC-23, and to interpret the protein in dentin formation and mineralization mechanism. The biological function to reveal the DMP and cell differentiation and dentin formation and pulpal repair, lay a biological basis to improve living pulp conservation effect. The contents and results are as follows: a. DMPI expression in human tooth development to take 4 Diao old fetus 3, take the upper and lower jaw, and preparation of human tooth development phases of the specimens, using immunohistochemical methods observed DMPI expression in different stages of human tooth germ. The results show, DMPI expression late bell stage secretory odontoblasts, former odontoblasts before ameloblasts and dental papilla cells weakly positive expression, transient expression in ameloblasts, that glaze matrix begins to form, but not formed mineralized secretory ameloblasts expressed fade to negative expression, but in the subsequent secretory ameloblasts. DMP expression of different periods and changes in human tooth development prompted the The DMPI plays an important role in odontoblast and ameloblast differentiation and maturation and dentin formation process, DMPI in ameloblasts expression mode can speculate DMPI start the enamel formation may exercise the role of cell signaling molecules. II. The DMPI different carious teeth immunolocalization through immunohistochemical method for the detection of human carious permanent teeth DMP expression changes, and semi-quantitative analysis of their expression levels. Found that negative expression in normal human permanent teeth pulp, DMP; DMP expression in carious permanent teeth are as follows: the superficial caries parts of odontoblast cytoplasm and predentin weak positive expression pulp caries and caries loss below the pulp cell proliferation significantly, the odontoblast layer obvious thickening, dyeing enhanced nuclear staining increased more than healthy permanent teeth. Pulp vacuolar degeneration of the odontoblast layer deep caries odontoblast-like cells, only a layer of flat, caries and deep caries group into odontoblasts and odontoblast-like cytoplasmic synapses positive expression the DMPI, while the Fourth Military Medical University, a master's degree thesis group reparative dentin of deep caries expression was strongly positive. Carious lesion under odontoblast expression of DMP, the protein is involved in the repair process pulpal. III. The parathyroid hormone human dental papilla cells DMPI expression by immunohistochemical methods observed parathyroid hormone a arathyroid of hormone, PTH) expressed in cultured human dental papilla cells DMP shadow. Primary cultured human dental papilla cells, stimulation of cells with different concentrations of parathyroid hormone. The results showed that of PTH can stimulate human dental papilla cells the DMPI the expression, induced DMPI mainly expressed in the cytoplasm, a dose dependent 0.3 pg plus L to stimulate optimal concentration. PTH can stimulate the human papilla cells DMPI odontoblast differentiation of dental papilla cells to have a certain role, may be one of the important mediators odontoblast differentiation. Moreover, stimulation of PTH, dental papilla cells to differentiate into odontoblast differentiation characteristics, and to promote the expression of the cell DMP, that PTH may be mediated odontoblast maturation and secretory activity induced DMP may be involved odontoblast differentiation process. IV. Antisense oligonucleotide on DMPI MDPC 3-cell alkaline phosphatase activity and mineralization capacity to target cells MDPC-23 cells stably expressing DMPI different concentrations DMPI antisense oligodeoxynucleotide (AS-ODN) blockers observed different time MDPC-23 cells ALP activity. 10 pmo ridiculed the different role of the AS-ODN inch between Taiwan compatriots DMPI expression, and to observe the impact of its continuous role 10d ability of the cell mineralization was detected by Western blot. Found that, with the normal group, and S-ODN group phase comparison, different concentrations of the AS-ODN be able to reduce the MDPC-23 cells ALP activity, of which 10 pmo where the AS-ODN reduce ALP activity is most noticeable with the (role of sd OD value of the minimum for the 0.3378t2 knife E). West

A Study on the Function of Dentin Matrix Protein 1 during Tooth Development and Dental Pulp Repair after Injury

Abstract

Related Dissertations