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Objective: Identification of the South China macaque red blood cell surface class ABO blood group, to determine the antigen enzyme alpha-galactosidase enzyme the macaques class B and macaque red blood cell morphology and function, understand the alpha-galactosidase enzyme The solution of red blood cells back to the lost receptors survival after as well as before and after reinfusion receptor macaque blood, urine biochemical changes in the evaluation of alpha-galactosidase enzymatic type B red blood cells of monkeys security. Methods: ① preparation macaque class ABO blood typing serum by improved absorption elution test screening from 30 South China macaque ABO blood group and select strong antigenicity two type A 3 B-type rhesus monkeys as experimental subjects . ② the macaque class B antigen recombinant alpha-galactosidase enzyme in vitro enzymatic, improved enzymatic effect of adsorption and elution test evaluation. (3) light microscopy and scanning electron microscopy before and after hydrolysis macaque red blood cell morphology, detection of the deformation, the cell membrane fragility, the hemolysis rate cholesterol, methemoglobin, acetyl cholinesterase, ATP ,2,3-DPG, P 50 < / sub> and red blood cell structure and function indicators for enzymatic erythrocyte membrane integrity, deformation capacity, oxygen carrying capacity, and the ability to metabolize. (4) enzymatic erythrocyte receptor (B1, A1, B2 → A2, B3 → B3) lost after fluorescently labeled (FITC) was measured before transfusion, 10 minutes after the transfusion, 30min, 60min when blood pressure, pulse, breathing. (5) Flow cytometry detection after enzymatic hydrolysis of red blood cells in the fluorescence intensity of the receptor in vivo, calculate the enzymatic hydrolysis of red blood cells in the body 24h survival, draw red blood half-life curve. (6) dynamic monitoring of receptor before and after different periods of reinfusion of blood, urine biochemical indicators. Results: (1) A type 3 (10%) were detected in 30 monkeys, B-9 (30%), AB-3 (10%), select 99263 (A1), 88113 (A2), 98107 ( B1), 95457 (B2) for the experimental group, 93281 (B3) for the control group. (2) two macaque B erythrocyte surface antigen by B alpha-semi-galactosidase after enzymatic hydrolysis, improved adsorption and elution test was negative. ③ enzymatic hydrolysis of erythrocyte consistent with the size, shape, refractive index, etc., are typical disk-shaped double-concave structure. The main function of red blood cells after alpha-galactosidase enzymatic hydrolysis indicators were normal. (4) receptor macaque blood pressure, pulse, respiration, no significant change (P> 0.05) compared with the pre-transfusion. ⑤ alpha-galactosidase enzymatic hydrolysis monkeys red blood cells in the body 24h survival was 84.6% and 68.1%, the half-life of 7d and 8d, 88% in the control group 24h survival of red blood cells in the body, the half-life for 13d. (6) receptor that monkey blood, urine and other biochemical parameters before transfusion was no significant difference (P gt; 0.05) Conclusion: (1) South China said the monkey red blood cell surface class of ABO blood group antigens. ② use. - The galactose past enzymatic hydrolysis method can clear class B known as monkey red blood cell surface antigen. No adverse effects on the morphology and function (3) Q-galactose past enzymatic hydrolysis symmetry monkey red blood cells, red blood cells lose the allotypes receptor does not occur transfusion reaction after hydrolysis. (4) infusion. Galactose past After enzymatic hydrolysis red blood cell receptor that monkey blood, urine and other biochemical markers had no significant effect.
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