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Efficacy of a Novel Plasma Jet as an Antibacterial Against Enterococcus Faecalis

Author: YangPing
Tutor: CaoYingGuang
School: Huazhong University of Science and Technology
Course: Clinical Stomatology
Keywords: Plasma plasma jet root canal disinfection Enterococcus faecalis biofilm
CLC: R187
Type: Master's thesis
Year: 2010
Downloads: 76
Quote: 0
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Abstract


Objective: This study aimed to explore a new and effective root canal disinfection method to improve the effectiveness of root canal disinfection, and to increase the success ratio of root canal treatment.Materials and methods: This study applied a nanosecond pulse-driven atmospheric pressure plasma jet to the culture dishes, the biofilm models of nitrocellulose membrane and the infectious models of the extracted single-rooted teeth with Enterococcus faecalis standard strains, respectively, so that the sterilization effectiveness and its influencing factors were explored. For example, the plasma jet was utilized with different time, conditions and approaches while in the same physical parameters. Whilst Staphylococcus aureus was compared with the sterilization effect as a control in the same conditions. The bacterial morphological changes were observed by scanning with transmission electron microscope to grope the mechanism of the sterilization. . And teeth in vitro after treatment were scanned by Micro-CT to get the 3D structure images, which could analyze the relationship between the morphology of the root canal system and the sterilization effectiveness in each sample.Results: The results revealed that the novel plasma jet worked on the Enterococcus faecalis culture dishes could form a significant inhibition zone, and bacterial number on biofilms and in isolated teeth decreased remarkably after treatment. While these results related close to the working time, conditions with plasma jet and the morphology of the root canal systems. Conclusion: This study provided a reliable, effective and simple disinfection method for root canal therapy. It not only carried out a platform for plasma applications, but also opened up the research content of "plasma medicine". In this study, non-equilibrium plasma technology was applied to oral medicine and made an important breakthrough in plasma medicine. And there is further work to be done to identify the reliability, effectiveness, safety and mechanism of sterilization with plasma jet.

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CLC: > Medicine, health > Preventive Medicine,Health > Epidemiology and Prevention of Disease > Disinfection
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