Dissertation > Excellent graduate degree dissertation topics show

Studies on Chromosomes and Mitoses of BmN Cells and Primary Culture of Bombyx Mori’s Embryonic Cells

Author: PuXiaoYing
Tutor: LuCheng；HongXiJun
School: Southwest Agricultural University
Course: Cell Biology
Keywords: Silkworm BmN cells Chromosome Mitosis Primary cultured
CLC: Q813.1
Type: Master's thesis
Year: 2003
Downloads: 116
Quote: 4
Read: Download Dissertation

Abstract

Insect cell culture technology as one of the foundations of Cell Engineering, is a valuable means of modern experimental biology, are widely used in various fields of biology, medicine and agriculture. Insect cells engineered cells in the baculovirus expression system, transformation and expression efficiency of the expression system is closely related to, therefore, the development of the study of insect cell expression system has far-reaching effect. The silkworm bioreactor baculovirus expression system with many advantages compared to other insect expression system has research significance. Silkworm cell lines because the efficiency of transformation and expression, its application is not as extensive, such as fall armyworm cell line. Strengthen the biological basis of theoretical research, to find more suitable for the baculovirus expression system silkworm cell lines engineered cells is the need of the current silkworm expression system research and development of. To force the the silkworm cells of basic biological research to be strengthened, and learn from the successful experience of other animals, by in vitro cell culture study is feasible and effective method. The same time, the silkworm in vitro cell culture studies carried out, not only can provide a good study system for the silkworm cell biology theory research, and also of great significance to the development and utilization of the silkworm resources. In this study, drawing on the basis of previous studies on the one hand, now widely used in the baculovirus expression system engineering cell-BmN cell chromosomes and mitosis research and analysis. Also by primary culture experiments silkworm embryonic cell line to explore and try and silkworm embryos original culture process cell and tissue types were observed, explore and research. 1 BmN cell mitosis and chromosome studies BmN cells is widely used in the silkworm molecular genetics, cell engineering, genetic engineering, and the baculovirus expression system silkworm cells, but little is known about the genetics and cell biology background. The BmN cells chromosomes and mitosis air drying method and cultured cell patch study found that: (1) the use of the air-dried prepared BmN cell chromosomes, cultured for 6 in the colchicine final concentration 0.1ug/ml of 28 ° C to 9h conditions can be obtained more metaphases, chromosome aggregation is relatively modest. (2) BmN cell chromosomes typical Lepidoptera chromosomes: the metaphase chromosomes short rod or granular, non-obvious centromere. BmN cells are highly aneuploid of highly polyploidy cell lines, the typical diploid rarely polyploidy group of up to 90%, the largest chromosome group of more than 500. (3) by air drying method by BmN cell metaphase chromosomes from meiotic metaphase chromosomes prepared by the silkworm gonads and embryos were tested granular morphology and short rod, is not very different. However, in a significant difference in the number of chromosomes. In addition, the difference between the length of chromosome is also very obvious. Meiotic metaphase chromosomes from silkworm gonads and embryo mitotic metaphase chromosomes, chromosome length was not significantly different, and BInN cells in metaphase chromosome length is very inconsistent, and the difference was very significant. K) BmN cell mitosis mainly to normal mitosis, but at the same time, there are times nuclear mitosis mitosis and similar constriction and splitting class mitotic nuclear phenomena. The silkworm embryo cells in primary culture door) In this study, on silkworm reversal embryonic material, half tissue explant and trypsin digestion the silkworm embryo cells in primary culture, primary adapt reversal embryonic cells The culture methods, i.e. trypsin the semi digestion method. Laid the foundation for the establishment of the new silkworm embryonic cell lines. Primary cultured cells and tissues block the in vitro growth phenomenon in vitro longest survival Damen months. Q), primary culture cell types during culture and changes a certain observation and research and found that appear in the primary culture of the silkworm embryos main cell and tissue types: epithelial-type cells and tissues, fibroblast diffuse organization and network organization, glandular tissue and fat-organization. There is also a small amount of common bean sprouts \

Related Dissertations

CLC: > Biological Sciences > Bioengineering ( Biotechnology ) > Cell Engineering > Cells, tissue culture techniques
© 2012 www.DissertationTopic.Net Mobile