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Mechanism on Metabolism of Isoflavones of Soybean [Glycine Max (L). Merri.]

Author: DongLiPing
Tutor: WuZhenLing
School: Southwest Agricultural University
Course: Biochemistry and Molecular Biology
Keywords: Soybean Isoflavones Phenylalanine ammonia lyase
CLC: Q946
Type: Master's thesis
Year: 2003
Downloads: 557
Quote: 3
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Abstract

Soybean isoflavones (Isoflavones Of Soybean) soybean body and especially seeds of a class of secondary metabolites accumulated much attention because it has a variety of biological functions. Starting the experiment from the free radical scavenging effect of flavonoids soy isoflavones as the main object of study, select the isoflavone content significantly four different varieties as experimental materials to study the distribution of of soy the whole growth isoflavones in tissue change and its accumulation laws; soybean seed development period, explore the relationship between the secondary metabolites of soy isoflavones organics metabolism, and its relationship with the antioxidant system; isoflavones in soy whole growth with its synthesis The relationship between the key enzyme - phenylalanine ammonia lyase (PAL), which the system up to reveal the mechanism of the metabolism of soy isoflavones, in order to fully understand the rational use of soy isoflavones provide theoretical and experimental evidence. The test results show that: flavonoids on superoxide anion radical (O ₂ ^{(?) ) and hydroxyl radical (· OH) has a significant role in clearing its clear The concentration of the effect of flavonoids in the reaction system has a clear dose-response relationship, i.e. the higher the concentration of the flavonoid compound in the reaction system, the free radical scavenging effect is better. Storage of isoflavones in soybean vegetative growth stage seed gradually by seed cotyledons, and then transferred to other organs (mainly leaves), different organs during plant growth and development in the metabolism of isoflavones. Distribution of the presence of tissue specificity of the vegetative growth period of isoflavones without species-specific differences between the organizations reached a very significant level: soybean seedling stage, the isoflavone content of the aerial parts is much higher than the underground part; differences in the seedling stage cotyledon the flavonoids far higher than other parts; the strong seedling period and flowering leaves isoflavones far than other organizations. During the reproductive growth of soybean isoflavones from vegetative organs (mainly leaves) accumulated to seed. Accumulation of isoflavones significantly seeds with the seed development process gradually increases, the correlation between the development of the number of days and moisture content reached a very significant level. With the increase in dry matter accumulation of isoflavones gradually increase to get higher isoflavone content of soy and then harvest must be fully mature seeds. Leaves isoflavones in soybean seed isoflavone content in the leaves far below the seed isoflavone content. Period of seed development, seed isoflavone content of leaves isoflavones content was negatively correlated reached a very significant level, indicating that during seed development, leaves isoflavones is gradually transferred to the seeds. The flavonoid metabolism studies on the mechanism of the soybean sector simply more strong vegetative growth stage before phenylalanine ammonia lyase activity changes in the organization that the strong seedling period and flowering, leaf PA L activity are far higher in the seedling stage, in other organizations, followed by leaf> stem> root, this difference in each period before the strong performance was obvious. And with the gradual growth of the seedlings from the seedling stage a strong seedling of a flowering periods, the isoflavones and PAL leaves are increasing, while the stems isoflavones and PAL is decreasing, a slight increase in the root or reduced change significantly. Very consistent with the changes in this trend isoflavones phenylalanine ammonia lyase, phenylalanine ammonia high activity, isoflavones content. This indicates that the The the vegetative stage phenylalanine ammonia lyase determines the level of the isoflavone content of, PAL is a key enzyme of the synthetic isoflavone. After flowering, seed development process, the downward trend in the seeds and leaves in the PAL activity were tested after the first rise, to 39 days to reach the maximum, and then declined. Show that the the phenylalanine ammonia level of activity synchronized with the seeds of developmental processes and metabolic PAL activity decreased isoflavones continues to increase, may isoflavones than as a product of phenylalanine ammonia-lyase said, there is a process of accumulation and relative lag period, its reasons for further analysis and research. Soybean seed development process was a significant positive correlation between the various varieties accumulation of isoflavones and protein content also increased with the increase in soluble protein in the seed isoflavone content. Early isoflavones increase of soluble sugar content increased mature isoflavones increase and decrease in soluble sugars in the seed formation. The relationship between the study of soy isoflavones and antioxidant system With the accumulation of isoflavones in the seeds in the seed formation in the early soybean seeds POD, SOD, CAT activity increased with seed isoflavone further accumulation activity decreased malondialdehyde content tended to decrease in the whole process of seed maturation. That soy isoflavones accumulated to make up for the reduction of other antioxidant enzymes and antioxidants, in order to ensure normal growth and development of soybean plants. POD isozyme analysis showed. POD isozyme of soybean leaves and roots in the process of accumulation of soy isoflavones, basically no new enzyme with the presence or absence of large changes, but the strength of the enzyme with change.}

Mechanism on Metabolism of Isoflavones of Soybean [Glycine Max (L). Merri.]

Abstract

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