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Study on the Interaction between the Probes of Supramolecular Cyclodextrins and DNA

Author: ZhangGuoMei
Tutor: ShuangShaoMin
School: Shanxi University
Course: Analytical Chemistry
Keywords: Cyclodextrin Molecular probes Role of Fluorescence quantum yield Ability to Inclusion Sub- system DNA double helix Neutral red Inclusion constant Phenothiazine
CLC: O651
Type: Master's thesis
Year: 2003
Downloads: 303
Quote: 0
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Abstract


Chapter 1: This chapter provides a brief overview of cyclodextrin (CD) and its derivatives in the life sciences, new progress, focuses on applications in molecular recognition in biological systems. And introduced DNA analysis of chemical research in progress, as well as the role of cyclodextrin supramolecular assembly of DNA elaborate. Chapter II: This chapter by fluorescence spectroscopy, UV-visible spectrophotometric study of β-CD, HP-β-CD, SBE-β-CD on the molecular recognition of neutral red, discussed in detail the factors that affect the process of inclusion. compare different cyclodextrin capacity and clathrate fluorescence quantum yield. The results showed that: the different cyclodextrins neutral red clathrate capability has selectivity, SBE-β-CD of the charge type cyclodextrin clathrate capacity is greater than the neutral cyclodextrin β-CD, HP-β-CD. Same cyclodextrin of neutral red body also has to identify the role of the three kinds the cyclodextrin are easier inclusion of neutral-type body and easy inclusion acidic type body. Inclusion complex inclusion constant is the greater, the greater the fluorescence quantum yield. Chapter 3: Fluorescence spectroscopy, UV-visible spectrophotometry, resonance light scattering technique to study the neutral red (NR) and the DNA and the neutral red - cyclodextrin supramolecular systems with DNA role. The results show that the neutral red interaction with DNA in two ways mainly depending on the molar ratio between (NR: DNA), i.e., R-value. When r> 2.5, when the DNA concentration is low, the system of NR present neutral-type body, with a unit of positive charge, while the DNA is negatively charged polyanion, between electrostatic interactions. And when a higher concentration of DNA, the DNA with NR induced NR generated with two units of positive charge protonated (acidic type body), but at the same time DNA also gradually protonated between the electrostatic role reduced, and thus interaction intercalation, the formation of DNA adducts. NR-CDs supramolecular systems with DNA, NR-CDs disintegration of the neutral body is protonated form adducts with DNA, NR-CDs supramolecular assembly and the role of DNA in the form of intercalation role. The NMR spectral further confirmed this conclusion. In fact, the NR-CDs supramolecular systems, cyclodextrin plays a carriage of the guest molecules fitted into the DNA double helix structure. Chapter IV: This article studies the β-cyclodextrin (β-CD) and its derivatives HP-β-CD, CM-β-CD, SBE-β-CD with safranine T for inclusion. Binding constants measured by fluorescence spectroscopy, compared four cyclodextrin inclusion ability of ST, and discussed in detail cyclodextrin concentration and system of medium acidity on the impact of the inclusion process, the inclusion mechanism . Chapter: small molecules to study the structure of the nucleic acid molecules, the status and function of the probe, the results of its research to the understanding of the pathogenic mechanisms of certain diseases and some medications (especially anti-cancer drug) treatment mechanism necessary information. This paper Supramolecular Systems cation safranine T interaction with DNA study calculated both the binding constant and the number of binding sites. The results show that: through supramolecular systems with DNA confirmed low concentrations, the role of DNA with ST DNA induced ST long range assembly on its surface, and in the DNA high concentrations, the the ST monomer molecules in the DNA intercalation the double helix, its fluorescence quantum yield increase. The sixth chapter of Chinese Abstract: In this paper, UV-visible spectroscopy to study the aggregation of methylene blue itself, explored the impact of of cyclodextrin their monomer and dimer balance. By fluorescence spectroscopy for the first time to study the neutral and charge-CD derivatives that hydroxypropyl p. The cyclodextrin (1_ [Pp · CD) and carboxymethyl p cyclodextrin (CM-p-CD) and methylene blue form a 1:1 inclusion complex, and compare the size of the clathrate constant. Charge type cyclodextrin the unique electrostatic inclusion performance thus easier inclusion of methylene blue molecules. Chapter VII: The combination of the first few chapters of the research work, explore the the phenothiazine compounds itself commonalities and common feature of the cyclodextrin and DNA role. Draw some rules: (1). Phenothiazine monomer cyclodextrin to clathrate forming HAD ratio of l: I supramolecular clathrate; (2). Cyclodextrin easier clathrate phenothiazine compound a neutral and anionic cyclodextrin clathrate object larger capacity than the neutral cyclodextrin; (3). Performance is, interaction with DNA, the phenothiazine compound is easily performed in the surface of the DNA molecule when the DNA concentration is low, long-distance assembly. When the DNA concentration is high, the monomer can be fitted into the DNA double helix structure, and improve the fluorescence quantum yield of the monomer; (4). When cyclodextrin. Phenothiazine supramolecular systems with DNA, the cyclodextrin actually played a carriage of the role of the guest molecules, so that the guest molecules more easily fitted into the DNA double helix.

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Fundamentals of Analytical Chemistry Theory
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