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Effects of the Huo-Xue-Shen-Shi-Fang on Expression of Fas, FasL, Bcl-2 and Bax in Liver Tissue of Rats with Hepatic Fibrosis

Author: ChenQunWei
Tutor: LuLiangWei
School: Zhejiang College of Traditional Chinese Medicine
Course: Basic Theory of Traditional Chinese Medicine
Keywords: The @ and blood circulation Shenshi side / therapeutic use Liver Cirrhosis, Experimental / TCD therapy Immunohistochemistry / diagnostic applications Rats , inbred Antigen , CD95 Proto-oncogene proteins c - bcl - 2
CLC: R259.7
Type: Master's thesis
Year: 2003
Downloads: 93
Quote: 0
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Abstract


Objective To observe the liver fibrosis in liver tissue of Fas, FasL, Bcl-2, Bax protein expression, as well as blood circulation Shenshi party intervention role to explore of promoting blood Shenshi side antifibrotic mechanism. The method 40% CCl in which 100 SD 4 oil induced rat model of liver fibrosis, the formation of moderate hepatic fibrosis SD rats were randomly divided into three groups, including the model control group, blood of Shenshi the square treatment group (blood Shenshi party group) and Fufangbiejiarangan liver slices positive control group (referred to as the soft liver slices group), and normal control group of 20. After 12 weeks of continuous treatment, detection of serum markers HA, the liver specimens do HE staining and picric acid day the wolf red staining (SR staining), liver fibrosis histological grade VI standard grading; immunohistochemistry liver tissue in Fas, FasL, Bcl-2, Bax expression, image analysis semi-quantitative scoring. Of serum HA test showed that levels of serum HA levels between the treatment groups, there were significant differences (P <0.05), the blood circulation Shenshi side group can significantly reduce serum HA, there was no significant difference between the soft liver slices group (P> 0.05). Pathological classification of liver fibrosis The blood circulation Shenshi group significant improvement in the degree of liver fibrosis model group significantly different (p <0.05), and soft liver slices between the groups was no significant difference (P> 0.05) . Compared with the normal control group, model control group rat liver tissue Fas and FasL Table Damien write to increase (P lt; 0.01), live blood Shenshi party group Fas and FasL expression compared with the model control group, no significant difference (P GT; 0.05). Model group Bd 2 and Bax than the normal group increased significantly (corpse lt; 0.01, P lt; 0.05); blood circulation Shenshi party group and soft liver slices group fibrous septa Bd 2 expression levels compared with model group decreased significantly (P are LT; 0.05), the expression of Bax was no significant change (P GT; 0.05); the blood circulation Shenshi party group fiber interval the Bax old el ratio was significantly higher (dead lt; 0 .05). Conclusion blood circulation Shenshi party has significant anti-experimental liver fibrosis; experimental liver fibrosis, liver tissue expression of Fas, FasL enhanced; FasL expression in the fibrous septa, Fas-mediated apoptosis and fibrous septa only cells are closely related; the blood circulation Shenshi party had no significant effect on the 'rat liver fibrosis induced expression of Fas, FasL; experimental liver fibrosis and liver Bd 2 and Bax Enhanced; blood circulation Wetting side treatment , can improve the the liver tissues fiber interval the Bax old cl value, blood circulation Shenshi side of the cells within the fibrous septa withered a death that may have a role in promoting; promote apoptosis of myofibroblasts (MFB) is the blood Shenshi party anti- one of the mechanisms of liver fibrosis.

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