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The Changes of Gastric Mucosal T Lymphocyte Subsets in Children Infection with Helicobacter Pylori

Author: LiZhongYue
Tutor: ChenJie
School: Zhejiang University
Course: Pediatrics
Keywords: Child Helicobacter pylori T lymphocyte subsets Gastric mucosa
CLC: R725.7
Type: Master's thesis
Year: 2004
Downloads: 42
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Abstract


Background \u0026 Objective】 Helicobacter pylori (Helicobacter pylori, H.pylori) is a kind of colonization in Gram-negative bacteria in human gastric mucosa with chronic gastritis, peptic ulcer, gastric adenocarcinoma and MALT lymphoma occurred close relationship. H.pylori pathogenic mechanism is very complex and not well understood, through its own power plant, the adhesion characteristics as well as a variety of virulence factors to cause direct damage to the gastric mucosa in H.pylori infection in gastric mucosa can be found in a large number of infiltration of lymphocytes and neutrophils. H.pylori infection by an increase in gastric secretion IgG plasma cells, local IgA, IgG concentration, cycle-specific antibodies, IgA concentration was significantly higher than that of non-infected persons, and in saliva, gastric juice, and feces can also detect high drop the degree of antibody. The study also confirmed that, H.pylori infection can directly activate or and indirectly stimulate neutrophil response induced by IL-8, a strong neutrophil response is caused by a mechanism of gastric mucosal injury. H.pylori infection in the body can occur after a strong humoral immune / inflammatory response, but H.pylori not be eradicated even last a lifetime. In recent years, more and more evidence to show that the cellular immune response of the host in the pathogenicity of H.pylori infection plays a key role, but H.pylori infection due to specific cellular immune response is currently no consistent conclusion, especially H.pylori infection-specific T lymphocytes of CD4 ~~ / CD8 different changes of Th1/Th2 subsets of different points of view. Children H.pylori infection in the cellular immune response is relatively small. In view of the majority of H.pylori infection in childhood, and children's lifestyle, H.pylori infection of short duration, the outcome of the infection, the pathological changes of gastric mucosa-related diseases at adults. Cellular immunity may be inconsistent with adults to understand the changes of T lymphocyte subsets in children H.pylori infection is of great significance for further clear the H.pylori naturally infected with the pathogenic mechanism. Therefore, this study using flow cytometry to detect H.pylori infection in children the local gastric mucosa and peripheral blood T lymphocyte subsets CD4 ~ / CD8 ~ changes compared with H.pylori H.pylori infection in children in order to understand T-lymphocyte subsets change, provide preliminary theoretical data to elucidate the mechanism of the immune pathogenic children H.pylori infection. Zhejiang University graduate thesis., Scoop Yin \Bo film group (the WAN edge Do ri nesativen ~~ al. mucos hurried from the sand Iorlwor) 8 cases, months {blade the tori-negative chronic superficial gastritis group (Wan Chuan. ri negative cb the old nic super identified five c 1gastritis Wan edge torl- million; CsG) 27 cases the edge the tori-positive chronic superficial gastritis (million edge Do ri positiveehronie sul Farm rficial gastritis, hall not to edge r the expansion CsG) 44 cases, Wan edge the tori-positive duodenal ulcer group (prop blade Do not ri Mao the sitive duodenal uleer, H. blade the IOr spoon U) 10 cases, all cases diagnosed by endoscopy, rapid urease test, histological examination, bacterial culture confirmed and take the antrum paid film as lymphocyte extract. The (Million edge of the 38 cases of children Do not r expanding the CSG group of 12 cases the Wan edge do not r wide the DU group of five cases, the Hall edge do not r-group, 21 cases) and extract heparin peripheral venous blood Zml. The positive diagnosis Wan edge not to ri infected tissue cultures positive or rapid urease test and histopathology bacteriological examination. Biopsy the antrum Bo film four set sterile Hank s solution, 1 mM EDTA (ethylenediamine tetraacetie aeid and lmM DTT (dithiothreitol) 37oC digestion lh abandoned epithelium containing 120U / m 11 collagenase Hank s solution at 37 ° C for digestion 3h, was discarded and the residue was collected after the lamina propria lymphocytes, lymphocytes were CD3 an FITC (fluoreseein an isothiocyanate), of CD4 a PE (R a Phyeoerthrin), CDS and a Pe especially P for (P ERID Association in ' IilorophyU a Q rotein) stained determination of T lymphocyte subsets in peripheral blood T lymphocyte subsets CD3 gating determination of trace amounts of whole blood by direct immunofluorescence. [11, stomach Bo film groups CD3 (%) cells the detection rates were: Hall edge the tor tumultuous or group 2.5 belly the of 0.81; Wan edge torl-CsG group Hall edge Do not r the wide CsG group .97 4.37 months; hall edge Do r the spoon U group of 6 2.18 3.4 vector; .71 5 states. 2 in the stomach salary membrane of en3 · cells in the eo4 · (o, o) ens (Xun's relative percentage Cn4 / Cns values ??were: 000 edge Zdr water or Group 23.2 belly 10.46 39.26 of disabilities 14.29,0.61 disabilities 0.22; the any edge ZOrfCsG group was 23.9 belly of 10.54,49.35 of disabilities 10.45,0.5 belly 0.23; hall is 40.28 Guests 11 .35,27.91 disabilities 8.84,1 .55 disabilities primiparous the wide CsG group 0.52; Hall blade before r Kwong u group was 29.46 Guests the of 10.38,52.04 disabilities 14.40,0.64 cut .36 compared with 000 blade zor Gang or group from the remuneration Do rl-csG / Wan edge ZOrl * DU the local stomach Bo film cDS cells The relative proportions of significantly increased (P lt; 0.05); tor Li Wan edge. compared Wan blade Do rl r / H. blade torl-CsG group * Cso gastric exclusion membrane the eD4 cells relative increase in the proportion of CDs cells were decreased Co4 / C ns was significantly increased by the Iraqi lt; 0.01); hall edge ri DU group cDS cells do not higher than the H blade tor the wide csG group, and the relative proportions of the relative proportions of CD4 cells, CD4 / C DS ratio of less than ten thousand still early ri CSG group Iraq lt; 0.01). 3, peripheral blood CD3 (%), C (%), CDs (%) and e / C DS were: Hall the edge IOrr group copies - hurried 65.27 Guests 5.60,33.00 5.73 of disabilities 5.69,23.52 disabilities, 1.50 Guests 0.57; the H edge IOrl * Cso groups to 67.79 disabilities 6.19,35.77 disabilities 8.62,22.94 disabilities 4.50,1.66 disabilities 0.69; the the Hall edge the lor spoon U group is 65.51 Guests 9.86,35.72 disabilities 4.67,27.13 disabilities 11.13,1.48 Sze 0.51. T lymphocyte subsets was no significant difference between the three groups. Conclusion】 Do ri infected children from the sand, the local Burgundy film abnormal presence of T-lymphocyte subsets, the WAN edge do not r wide CsG CD4 lymphocytosis main, and associated with duodenal ulcer by CDS lymphocytosis based, both immune response is not consistent. Local gastric exclusion membrane changes of T lymphocyte subsets, Children Do ri infection Wan blade systemic changes of T lymphocyte subsets in no change?

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CLC: > Medicine, health > Pediatrics > Children within the science > Pediatric digestive and abdominal diseases
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