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While a large range of hepatectomy or treatment of severe liver trauma, usually in need of liver blood flow is temporarily blocked. Liver ischemia and hypoxia reached a certain extent, and time will cause damage to the liver cells, but restore the hepatic blood flow, liver cells instead was a more serious injury than ischemia and hypoxia. Ischemic injury but seek not to make the recovery of the liver blood supply tissue recovery functions, but heavier the injury phenomenon, known as hepatic ischemia-reperfusion injury (of hepatic ischemia. These harmful aspects of this returning blood flow have been termed reperfusion injury, HIRI). In recent years, the hepatic ischemia-reperfusion injury mechanism and prevention has become a hot research. That the mechanism of hepatic ischemia-reperfusion injury: the generation of oxygen free radicals, the imbalance in the concentration of nitric oxide (Nitric Oxide, NO) and endothelin (Endothelin, ET), the participation of cytokines and microcirculation obstacle . Based on hepatic ischemia-reperfusion injury prevention drugs gradually in depth, a lot of research has proven drugs, such as vitamin E, vitamin C, coenzyme Q, biologics SOD has a strong anti-tissue and organ ischemia-reperfusion injury . In the country, for the prevention and treatment of tissue and organ ischemia reperfusion traditional Chinese medicine preparation also fruitful. Shenfu Injection from the Shenfu Decoction woman recipe \alkaloids, modulation of immune function excitement pituitary - adrenal cortex, improve microcirculation of the role. This experiment to establish the rabbit hepatic ischemia-reperfusion model, determination of blood alanine aminotransferase (Alanine Aminotransfemse, ALT), aspartate aminotransferase Journal of Zhejiang University (the Aspartate Aminotransferase AsT), lactate dehydrogenase (Lactate Dehydro the nase, LDH) superoxide dismutase (s eroxide Dismutase soD), malondialdehyde (Maleic Dialdehyde, MDA), tumor necrosis factor (Tumor Neerosis Faetor a, TNF a) interleukin-10 (Interleukin an 10, of IL 10), the activity of NO and ET, observed liver cell morphological changes by comparing Shenfu injection of these indicators, in order to explore the Shenfu Injection ischemia-reperfusion injury in the liver protective effect and its mechanism. MATERIALS AND METHODS Animal Center of Zhejiang University School of Medicine in New Zealand white rabbits were 27, male weight 1.8 2.skg, (average 2.2kg). Routine preoperative fasting 12h, can not help but water. New Zealand white rabbits with thiopental 1oomg'kg'' intramuscular injection, a marginal ear vein after anesthesia placed arterial catheter (22G) for intraoperative infusion injection. Take the neck midline incision, separation of the right carotid artery, indwelling arterial catheter, to prepare for blood collection. From the midline incision abdominal wall into the abdominal cavity, free liver doors, the left lobe of the liver at the top of the caudate lobe free, the middle of the right middle lobe and square leaves the blood vessels and bile ducts, blood vessels arteries clipping, inflow occlusion, 45min after the release artery clip refilled the 45min formation of HI guilty model. The experimental animals were randomly divided into three groups, each 9. The control group (n = 9), Group A: Only free liver exposed liver \(n = 9), Group C: before ischemia 10min from the marginal ear vein injection maintained until Shenfu Injection (Yaan Sanjiu Pharmaceutical Co., Ltd., 10ml'k, dissolved in 5% glucose injection to looml) the end of surgery 10 minutes before the beginning of the A and group B in ischemia from the marginal ear vein infusion volume of 5% glucose injection, to maintain the same time three groups of rabbits were before ischemia 10min, ischemia 45min and reperfusion 45min blood was collected from the carotid artery, each time sml, determination of ALT, AST, LDH, SOD, MDA, INF a, IL 10, NO, ET. reperfusion 45min take a little left lobe of liver tissue, fixed in 10% formalin , embedded in paraffin, sliced ??(6 feet) HE staining under light microscopy. Zhejiang University, a master's degree thesis SPSSll.5 statistical software for statistical analysis, all data are teeth disabilities s group samples between the two groups were compared t-test, P lt; 0.05 as statistically significant ALT, AST, LDH, SOD, MDA, INF - a, IL 10, NO, ET parameters by Pearson. 1. liver function Results: A preoperative 45min after ischemia and reperfusion 45min serum ALT, AST, LDH concentration was no significant difference in group B serum ALT, AST, LDH concentration was significantly higher in the liver ischemia for 45 min reperfusion after 45min C group liver ischemia for 45 min after further elevated serum ALT, AST, LDH concentration also increased, but reperfusion 45min serum ALT, AST, LDH concentration was significantly lower than that in group B (P lt; 0.05) in the same period. 2.SOD results: B plasma SOD activity in the liver 45 min of ischemia and reperfusion 45min gradually reduce C plasma SOD activity is not significantly decreased, especially reperfusion 45min plasma SOD activity was significantly higher than in group B (P lt; 0.05) in the same period. the 3.MDA results: group B plasma MDA content in the liver ischemia 45 and n significantly higher reperfusion 45min rose more significantly. obvious plasma MDA levels increased in group C, reperfusion 45min MDA level significantly lower than that in group B earlier levels (P lt; 0.01). 4.TNF result: B group plasma TNF-a concentration in the liver ischemia 45min and reperfusion 45min gradually increased C group liver ischemia 45min plasma concentrations of TNF - a slightly higher ; reperfusion 45min plasma TNF-a concentration instead declined significantly lower than the level of the same period and group B (P lt; 0.01). S.IL 10 results: B plasma IL-10 concentration in liver ischemia 45min reperfusion 45min decreased significantly and no significant decline in group C plasma IL-10 concentration reperfusion 45min of plasma IL-10 concentration was significantly higher than the same level of group B (P lt; O, 05) Journal of Zhejiang University 6.NO Results: B plasma NO content in the liver ischemia 45min and decreased significantly after 45min reperfusion group C ischemia 45min and reperfusion 45min plasma NO content also declined, but significantly higher than in group B (P lt reperfusion 45min ago levels ; 0.05). 7.ET results: B plasma ET content in the liver ischemia 45min and increased perfusion 45min. the C group Ischemic 45 plasma ET content was increased, instead dropped after reperfusion 45min ischemia below the level before the two have a significant difference (P lt; 0.05). 8.B group
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